河北中医药学报2026，Vol.41Issue(1)：8-14,7.
基于Ca2+膜电位调节作用探讨大豆卵磷脂对小鼠精子质膜完整性的影响
Effect of Soybean Lecithin on Plasma Membrane Integrity of Mouse Sperm via Its Regulatory Effect on Ca2+Membrane Potential
摘要
Abstract
Objective:By adding different doses of soybean lecithin(SL),we analyzed the relationship between SL and cholester-ol content in the sperm plasma membrane,and explored the cryoprotective effect of SL on mouse sperm based on its regulatory effect on calcium ion(Ca2+)membrane potential.Methods:Sperm samples were collected from 40 healthy 8-week-old male mice and randomly divided into five groups:the blank control group(BC group),model control group(MC group),low-dose group(LD group),medi-um-dose group(MD group),and high-dose group(HD group).No treatment was administered to the BC group,while only cryopro-tective agent(CPA)was added to the MC group;the other three groups were treated with CPA containing different concentrations of SL(5 g/L,10 g/L,15 g/L),respectively.After cryopreservation and thawing,following indicators of sperm in each group were detected and analyzed:sperm motility,viability rate,straight-line velocity,curvilinear velocity,cholesterol content,Ca2+content,sperm plas-ma membrane integrity rate,and sperm membrane potential,and the cryoprotective effect of SL on mouse sperm was investigated.Results:Compared with the BC group,the MC group exhibited significant increases in sperm motility,viability rate,straight-line velocity,curvilinear velocity,plasma membrane integrity rate,Ca2+concentration and membrane potential(P<0.05),with no signifi-cant difference in T-CHO content(P>0.05).Compared with the MC group,the MD group showed significant increases in sperm mo-tility,viability rate,straight-line velocity,curvilinear velocity,T-CHO content,plasma membrane integrity rate,Ca2+concentration and membrane potential(P<0.05);only the plasma membrane integrity rate,Ca2+concentration and membrane potential were increased in the LD group(P<0.05);and only the T-CHO content was elevated in the HD group(P<0.05).Conclusion:SL at a concentration of 10 g/L can optimally regulate sperm Ca2+influx,stabilize membrane potential to protect the integrity of sperm plasma membrane,thereby improving sperm quality and achieving a cryoprotective effect.关键词
大豆卵磷脂/精子冷冻/精子活力/精子存活率/钙离子/膜电位/精子质膜完整性Key words
Soybean lecithin/sperm cryopreservation/sperm motility/sperm viability/Ca2+/membrane potential/sperm plasma membrane integrity分类
医药卫生引用本文复制引用
黄鹏诚,李欢,陈胜辉..基于Ca2+膜电位调节作用探讨大豆卵磷脂对小鼠精子质膜完整性的影响[J].河北中医药学报,2026,41(1):8-14,7.基金项目
江西省"科技+中医药"联合计划项目(2023KZI01007) （2023KZI01007）
江西省中医药管理局科技计划重点项目(2023Z026) （2023Z026）
江西省中医药重点研究室(第二批)建设项目-中医男科疾病重点研究室(KP202102007) （第二批）
