河南农业科学2026，Vol.55Issue(2)：59-69,11.DOI:10.15933/j.cnki.1004-3268.2026.02.007
β-氨基丁酸浸种对盐胁迫下杜仲种子萌发及幼苗生理代谢的调控
Regulation of Seed Germination and Seedling Physiological Metabolism of Eucommia ulmoides under Salt Stress by Soaking with β-Aminobutyric Acid
摘要
Abstract
To alleviate the negative effects of salt stress on the germination and growth of Eucommia ulmoides seeds,salt free treatment was used as the control(CK)to investigate the effects of soaking E.ulmoides seeds in different concentrations of β-aminobutyric acid(BABA)(5,10,20,40,80,160 mmol/L,denoted as BA1,BA2,BA3,BA4,BA5,BA6)under 100 mmol/L salt stress(SS)on seed germination and seedling growth,gas exchange parameters,key enzymes and antioxidants in the ascorbic acid(AsA)-glutathione(GSH)cycle,and hormone metabolism.The results showed that under salt stress,the germination,seedling growth,and physiological processes of E.ulmoides seeds were inhibited,and the AsA-GSH cycle and hormone metabolism balance were disrupted.Different concentrations of BABA treatment could alleviate the inhibition of salt stress on E.ulmoides seed germination and enhance the salt resistance of seedlings.Compared with SS treatment,SS+BA2 treatment increased the germination rate,germination vigor,germination index,and vitality index of E.ulmoides seeds by 74.1%,85.6%,87.9%,and 84.3%,respectively.The height,leaf area,and fresh weight(aboveground and root systems)of seedlings showed varying degrees of increase.Among the various concentrations of BABA treatment,SS+BA2 treatment had the best effect on improving the content of chlorophyll a,chlorophyll b,total chlorophyll,and carotenoids in seedlings,increasing by 49.3%,112.5%,64.2%,and 54.8%respectively compared to SS treatment.The net photosynthetic rate(Pn),transpiration rate(Tr),and stomatal conductance(Gs)in gas exchange parameters increased by 51.8%,88.9%,and 44.7%respectively,while the intercellular CO2 concentration(Ci)decreased by 46.5%.Under salt stress,the soaking with different concentrations of BABA increased the activities of ascorbate peroxidase(APX),glutathione reductase(GR),monodehydroascorbate reductase(MDHAR),dehydroascorbate reductase(DHAR),and the contents of AsA and GSH in E.ulmoides seedlings.Compared with SS treatment,SS+BA2 treatment increased the activities of APX,GR,MDHAR,DHAR,and the contents of AsA and GSH by 142.7%,126.0%,167.2%,169.4%,103.8%,and 132.3%,respectively;the content of indole-3-acetic acid(IAA)reached its maximum value of 39.2 ng/g in SS+BA2 treatment,and the content of gibberellin(GA3)and zeaxanthin nucleoside(ZR)also showed an increasing trend.In conclusion,treating E.ulmoides seeds with 10 mmol/L β-aminobutyric acid showed the most significant effect in alleviating the negative effects of salt stress.关键词
杜仲/β-氨基丁酸/盐胁迫/叶绿素/气体交换参数/激素Key words
Eucommia ulmoides/β-aminobutyric acid/Salt stress/Chlorophyll/Gas exchange parameter/Hormone分类
农业科技引用本文复制引用
马琦洋,黄雪莲..β-氨基丁酸浸种对盐胁迫下杜仲种子萌发及幼苗生理代谢的调控[J].河南农业科学,2026,55(2):59-69,11.基金项目
浙江省级创业训练计划项目(S202310344003X) （S202310344003X）
2023年浙江省新苗人才计划项目(2023R410004) （2023R410004）
