海洋科学2025，Vol.49Issue(8)：63-70,8.DOI:10.11759/hykx20250305002
基于环境DNA与影像技术的卡罗琳海山八放珊瑚多样性研究
Integrating environmental DNA and imaging techniques to study octocoral diversity on the Caroline Seamount
摘要
Abstract
Octocorals are a group of macrobenthos,key to deep-sea seamount ecosystems and play a crucial role in maintaining deep-sea biodiversity.However,due to technological limitations in traditional deep-sea survey methods,studies on the octocoral diversity in deep-sea seamounts remain insufficient.This study combined environmental DNA(eDNA)metabarcoding with imaging techniques to comprehensively analyze octocoral diversity on the Caro-line Seamount and evaluate the feasibility of eDNA technology in seamount surveys.eDNA analysis detected 65 molecular species and 19 genera,belonging to 11 families and 2 orders.In contrast,imaging techniques identified 29 morphospecies and 9 genera,belonging to 6 families and 2 orders.Both methods detected deep-sea octocoral groups,such as Chrysogorgiidae,Keratoisididae,Primnoidae,and Acanthogorgiidae,common to the tropical West-ern Pacific.They also revealed variations in the community structure and species composition.eDNA technology was more sensitive in detecting cryptic and rare species,whereas imaging methods exhibited greater advantages in quantitative analysis and morphological characterization.This study thus demonstrates that integrating eDNA and imaging techniques can significantly enhance the comprehensiveness and reliability of deep-sea octocoral diversity assessments,providing a new technical approach for deep-sea biodiversity research.关键词
环境DNA/八放珊瑚/卡罗琳海山/宏条形码技术/影像技术Key words
environmental DNA/Octocorallia/Caroline Seamount/metabarcoding/imaging techniques分类
资源环境引用本文复制引用
郑小宇,徐雨,徐奎栋,类彦立,詹子锋..基于环境DNA与影像技术的卡罗琳海山八放珊瑚多样性研究[J].海洋科学,2025,49(8):63-70,8.基金项目
国家自然科学基金(42176128、41930533、42306102) （42176128、41930533、42306102）
中国科学院战略性先导科技专项(XDB42000000) the National Natural Science Foundation of China,Nos.42176128,41930533,42306102 （XDB42000000）
Progress of Strategy Priority Research Program(Category B)of Chinese Academy of Sciences,No.XDB42000000 （Category B）
