菌物学报2026，Vol.45Issue(2)：98-109,12.DOI:10.13346/j.mycosystema.250175
基于全基因组开发小麦矮腥黑粉菌特异性分子标记
Screening of molecular markers specific for Tilletia controversa based on the whole genome sequence
摘要
Abstract
Wheat dwarf smut,caused by Tilletia controversa(TCK),is a dangerous fungal wheat disease that is highly destructive and difficult to eradication,possessing a significant impact.The disease is known as"No.1 disease of wheat",and is listed as a quarantine pest by European Plant Protection Organisation(EPPO),Australia,China and other countries/regions and organizations.Due to difficulties for distinguishing TCK from other closely related species such as Tilletia laevis in levels of morphological characteristics and molecular biology,misdiagnosis easily happen in quarantine inspections.In this study,TCK-specific molecular markers were developed at the genomic level,and four candidate genes,namely OG0008430,OG0008601,OG0008603 and OG0008610,were screened.Eight pairs of primers and four probes based on these four candidate genes were designed.Validation of combinations among the primers and probes were verified,and two sets of"triple+single"real-time fluorescence PCR methods were established.This combined method can effectively distinguish target species(TCK)from closely related species,achieving accurate identification of TCK,and it can also be used to distinguish different species within the genus Tilletia.关键词
小麦矮腥黑粉菌/多重实时荧光PCR/分子标记/全基因组/检测方法Key words
Tilletia controversa/multiple real-time fluorescence PCR/molecular marker/genome/detection method引用本文复制引用
高瑞芳,赵鹏,汤怡静,刘宵宵,徐欣宇,郑铭森..基于全基因组开发小麦矮腥黑粉菌特异性分子标记[J].菌物学报,2026,45(2):98-109,12.基金项目
海关总署科研项目(2023HK001) （2023HK001）
西藏自治区科技计划项目重点研发计划项目(XZ202201ZY0011N) This work was supported by the Scientific Research Projects of the General Administration of Customs(2023HK001)and the Key Research and Development Projects of the Xizang Autonomous Region Science and Technology Plan(XZ202201ZY0011N). （XZ202201ZY0011N）
