菌物学报2026，Vol.45Issue(2)：117-134,18.DOI:10.13346/j.mycosystema.250058
巨大侧耳Zn(Ⅱ)2Cys6型转录因子在Cd2+胁迫下的表达模式及其功能预测
Expression profiling and functional prediction of Zn(Ⅱ)2Cys6-type transcription factors in Pleurotus giganteus under cadmium stress
摘要
Abstract
Zn(Ⅱ)2Cys6-type transcription factors play pivotal regulatory roles in various biological processes in fungi,including primary and secondary metabolism,stress responses,and cell division.In this study,a hidden Markov model(HMM)was employed to screen the whole genome of Pleurotus giganteus,and 67 genes belonging to the Zn(Ⅱ)2Cys6-type transcription factor family were identified.Motif analysis revealed distinct sequence features among the family members.Phylogenetic analysis further classified these 67 genes into four major clades.Based on transcriptomic data,53 family genes with differential expression were identified under 10 μmol/L Cd2+stress for 3 h,6 h,and 12 h,as well as under 100 μmol/L Cd2+stress for 3 h,6 h,12 h,and 24 h.By using weighted gene co-expression network analysis(WGCNA),gene co-expression network topological analysis,and GO/KEGG enrichment analysis of co-expressed genes,the regulatory functions of some differentially expressed family genes were predicted.This study provides a scientific basis for further understanding of the role of Zn(Ⅱ)2Cys6-type transcription factor family in the response mechanisms of P.giganteus to cadmium stress.关键词
侧耳属/锌簇型转录因子/基因家族/转录组Key words
Pleurotus/zinc cluster transcription factor/gene family/transcriptome引用本文复制引用
刘悦,宋学超,张娜,张蕊,李维焕,程显好,杨树德..巨大侧耳Zn(Ⅱ)2Cys6型转录因子在Cd2+胁迫下的表达模式及其功能预测[J].菌物学报,2026,45(2):117-134,18.基金项目
山东省现代农业产业技术体系食用菌创新团队遗传育种岗位(SDAIT-07-03) （SDAIT-07-03）
山东省科技型中小企业创新能力提升工程项目(2021TSGC1233,2023TSGC900) This work was supported by the Genetic Breeding Position in Edible Mushroom Innovation Team of Shandong Modern Agricultural Industrial Technology System(SDAIT-07-03)and the Science and Technology-based Small and Medium-sized Enterprises Innovation Capacity Enhancement Project of Shandong Province(2021TSGC1233,2023TSGC900). （2021TSGC1233,2023TSGC900）
