内蒙古农业大学学报（自然科学版）2026，Vol.47Issue(2)：8-15,8.DOI:10.16853/j.cnki.1009-3575.2026.02.002
18种石竹属材料叶绿体DNA和线粒体DNA的SSR分析及其与花表型的关联性初探
SSR Analysis of Chloroplast DNA and Mitochondrial DNA of 18 Species of Dianthus and Its Correlation with Floral Phenotypes
摘要
Abstract
This study investigated the genetic diversity and relationships of the chloroplast and mitochondrial genomes in plants,fo-cusing on Dianthus chinensis cultivars,which exhibit a wide range of petal colors.A total of 100 SSR primers selected from a previ-ous experiment were used to analyze the chloroplast DNA and mitochondrial DNA of 12 cultivars of Dianthus chinensis,along with 6 other plant species of Dianthus.In the chloroplast DNA analysis,amplified bands were polymorphic by 30 SSR primer pairs.There were two major clusters at a genetic similarity coefficient of 0.50.Cluster I included DCPS from 1 to 3,Dca,Dsu,Dba-Sooty,Dba-Red,and Dkn,had no white sections in their petals.Cluster II comprised SCBS from 1 to 3,Dch-UW,Dch and BCBS from 1 to 5,had white sections in their petals except for Dch.In mitochondrial DNA analysis,amplified bands were polymorphic by 28 SSR pairs.There were two major clusters at a genetic similarity coefficient of 0.50.Cluster I included DCPS from 1 to 3,Dca,Dsu,Dch,BCBS from 1 to 5,SCBS from1 to 3 and Dch-UW,had large sizes of petals.Cluster II consisted of Dkn,Dba-Sooty and Dba-Red,had small sizes of petals.The genetic similarity coefficients among the D.chinensis cultivars ranged from 0.46 to 0.73,while the coefficients between D.chinensis cultivars and other Dianthus species ranged from 0.45 to 0.68,based on SSR analysis of chlo-roplast and mitochondrial DNA.关键词
石竹属/叶绿体/线粒体/SSR/聚类分析Key words
Dianthus/Chloroplast/Mitochondrial/SSR/Cluster analysis分类
农业科技引用本文复制引用
姜慧慧,王艺涵,贺学勤..18种石竹属材料叶绿体DNA和线粒体DNA的SSR分析及其与花表型的关联性初探[J].内蒙古农业大学学报（自然科学版）,2026,47(2):8-15,8.基金项目
国家自然科学基金项目(31960611) （31960611）
内蒙古自治区自然科学基金项目(2021MS03041) （2021MS03041）
