西华大学学报（自然科学版）2026，Vol.45Issue(2)：64-73,10.DOI:10.12198/j.issn.1673-159X.5904
Rhizopus oryzae脂肪酶@改性二氧化硅催化醇解法富集鱼油中EPA和DHA
Enrichment of EPA and DHA in Fish Oil via Enzymatic Alcoholysis by Rhizopus oryzae Lipase@Modified Silica
左东杰 1蒋曹奇 1李倩 1苟兴莉 1徐晓语 1曹茜1
作者信息
- 1. 西华大学食品与生物工程学院,四川 成都 610039
- 折叠
摘要
Abstract
This study employed nano-SiO2 modified with trimethoxyphenylsilane(TMPS)and trimeth-oxyoctylsilane(TMOS)as carriers for the immobilization of Rhizopus oryzae lipase(ROL).The effects of the differently modified carriers on the enzymatic alcoholysis of fish oil for enriching eicosapentaenoic acid(EPA)and docosahexaenoic acid(DHA)were investigated.By measuring enzyme activity and analyzing reaction products,the loading efficiency of the two immobilized enzymes(ROL@SiO2-TMPS and ROL@SiO2-TMOS)and their enrichment effects on EPA and DHA were compared.The results show that TMOS provides superior hydrophobic modification of SiO2,significantly enhancing enzyme loading(92.08%).During the alcoholysis reaction,ROL@SiO2-TMPS exhibites better enrichment for EPA(EPA content reaches 1.93 times the initial level at 2 h,with a recovery rate of 98.62%),while ROL@SiO2-TMOS showes better enrichment for DHA(DHA content reaches 1.98 times the initial level at 2 h,with a recovery rate of 98.25%).The enriched EPA and DHA are primarily distributed in diglycerides.Simultan-eously,the by-product fatty acid ethyl esters(FAEEs)generated in the reaction were rich in myristic acid,palmitic acid,and palmitoleic acid(sum>80%),which can be utilized as biodiesel.The findings of this study achieves efficient enrichment of EPA and DHA coupled with high-value utilization of by-products,providing an effective approach for the comprehensive utilization of fish oil resources.关键词
脂肪酶/二氧化硅/鱼油/二十碳五烯酸/二十二碳六烯酸Key words
lipase/silica/fish oil/EPA/DHA分类
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左东杰,蒋曹奇,李倩,苟兴莉,徐晓语,曹茜..Rhizopus oryzae脂肪酶@改性二氧化硅催化醇解法富集鱼油中EPA和DHA[J].西华大学学报（自然科学版）,2026,45(2):64-73,10.
