中国森林病虫2026，Vol.45Issue(1)：1-11,11.DOI:10.19688/j.cnki.issn1671-0886.20250066
耐寒近芽孢杆菌Rhs-L31的杀线活性及其对松材线虫病的防效
Nematicidal activity of Peribacillus frigoritolerans Rhs-L31 on Bursaphelenchus xylophilus and control effect against pine wilt disease
摘要
Abstract
To explore the nematocidal activity and biocontrol potential of strain Rhs-L31 against Bursaphelenchus xylophilus,the strain Rhs-L31 was identified by morphology and 16S rDNA sequence analysis.The nematocidal activity,reproductive inhibition ability of the strain Rhs-L31 against B.xylophilus and effect on lipid droplet morphology in B.xylophilus bodies were determined by the treatment of fermentation broth and fermentation filtrate.The control effect of Rhs-L31 fermentation broth on Pinus tabuliformis seedlings and the effects of Rhs-L31 fermentation broth on the activities of related defense enzymes in P.tabuliformis were evaluated by pot experiment.The results showed that strain Rhs-L31 was preliminarily identified as Peribacillus frigoritolerans.Fermentation broth and fermentation filtrate exhibited strong nematocidal activity against B.xylophilus and the corrected mortality was both as high as 100%at 24 h after treatment.The corrected mortality of B.xylophilus was 76.90%and 72.60%at 24 h after treatment with Rhs-L31 fermentation broth and fermentation filtrate diluted five times,respectively.Fermentation broth and fermentation filtrate significantly reduced the reproduction coefficient and egg number of B.xylophilus.Oil red O staining showed that Rhs-L31 fermentation broth and fermentation filtrate could destroy the lipid droplet morphology in B.xylophilus;pot experiments showed that fermentation broth injection treatment could significantly reduce the occurrence of pine wilt disease,and the control effect was 52.08%-83.33%for 14-35 days after treatment.Rhs-L31 fermentation broth could significantly reduce the density of B.xylophilus in P.tabuliformis seedlings,and the density of B.xylophilus in the prevention group was significantly lower than that in the control group.Rhs-L31 enhanced the activities of polyphenol oxidase,catalase,superoxide dismutase and peroxidase in P.tabuliformis seedlings against B.xylophilus.The results revealed that strain Rhs-L31 had potential for biocontrol of pine wilt disease,which could provide a reference for the development of efficient and green prevention and control technology of pine wilt disease.关键词
松材线虫病/生防细菌/耐寒近芽孢杆菌/杀线活性/生防效果Key words
pine wilt disease/biocontrol bacterium/Peribacillus frigoritolerans/nematicidal activity/biocontrol effect分类
农业科技引用本文复制引用
薛娟娟,梁超琼,王婷,孙孟娇,唐光辉,李培琴..耐寒近芽孢杆菌Rhs-L31的杀线活性及其对松材线虫病的防效[J].中国森林病虫,2026,45(1):1-11,11.基金项目
陕西省林业科技创新重大专项"松材线虫病绿色防控新技术开发与集成示范"(SXLK2024-0101) （SXLK2024-0101）
陕西省林业科技创新重点专项"松材线虫病的生防菌挖掘及其菌剂研制"(SXLK2023-02-40) （SXLK2023-02-40）
