中国森林病虫2026，Vol.45Issue(1)：12-17,6.DOI:10.19688/j.cnki.issn1671-0886.20250045
杀松材线虫细菌HEBAU PA-7鉴定及其发酵滤液稳定性分析
Identification of Bursaphelenchus xylophilus-killing bacterium HEBAU PA-7 and stability analysis of its fermentation filtrate
摘要
Abstract
To identify the biocontrol bacteria strain HEBAU PA-7 against Bursaphelenchus xylophilus,its taxonomic status was determined by morphological observation and molecular biology techniques,and the nematicidal activity and stability of its fermentation filtrate were evaluated by direct contact method.The results indicated that the strain colonies were yellow-green,circular,and vary in size,with rod-shaped bacterial cells and polar flagella.HEBAU PA-7 was the most closely related to Pseudomonas aeruginosa,with a homology up to 99.93%.The fermentation filtrate of strain exhibited strong nematocidal activity against B.xylophilus,achieving a corrected mortality rate of 100%after 72 hours of treatment with a 10-fold dilution.The nematocidal activity of the fermentation filtrate was unaffected by temperature,proteinase K or ultraviolet radiation,but was sensitive to strong acids and alkalis,with an optimal pH value range of six to eight.The fermentation filtrate of strain HEBAU PA-7 demonstrated strong nematocidal activity and environmental stability,providing a reference for the development and utilization of this strain.关键词
铜绿假单胞菌/松材线虫/发酵滤液/杀线活性/稳定性Key words
Pseudomonas aeruginosa/Bursaphelenchus xylophilus/fermentation filtrate/nematocidal activity/stability分类
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李佳琪,付宁宁,肖杰,张桉淇,高鸿鑫,李会平..杀松材线虫细菌HEBAU PA-7鉴定及其发酵滤液稳定性分析[J].中国森林病虫,2026,45(1):12-17,6.基金项目
河北省研究生创新资助项目"松材线虫与松树枯萎病木伐桩内生菌互作机制探究"(CXZZBS2024086) （CXZZBS2024086）
