中国森林病虫2026，Vol.45Issue(1)：60-64,5.DOI:10.19688/j.cnki.issn1671-0886.20250033
基于TaqMan探针实时荧光定量PCR的白蜡鞘孢菌分子鉴定技术
Molecular identification technology of Hymenoscyphus fraxineus based on TaqMan qPCR
摘要
Abstract
To rapidly and accurately detect Hymenoscyphus fraxineus,pathogenic fungus of ash dieback,internal transcribed spacer(ITS)and translation elongation factor 1-α(TEF1)genes were selected as targets for designing specific primers and TaqMan probes.A TaqMan probe-based qPCR detection system was established for target strains,and assay was optimized by adjusting annealing temperatures and concentrations of primers and probes.The results showed that TaqMan qPCR system could specifically amplify target genes of target strain,and minimum detection concentration of DNA was 1.0 pg/μL.The detection system could enable efficient quarantine identification and epidemic monitoring of H.fraxineus,and provide technical services and support for prevention and control of invasive pests at ports and protection of ecological security.关键词
TaqMan探针/实时荧光定量PCR/白蜡鞘孢菌/灵敏度Key words
TaqMan probe/real-time fluorescence quantitative PCR/Hymenoscyphus fraxineus/sensitivity分类
农业科技引用本文复制引用
杨春光,李辉,刘亚乔,薛腾,李鑫..基于TaqMan探针实时荧光定量PCR的白蜡鞘孢菌分子鉴定技术[J].中国森林病虫,2026,45(1):60-64,5.基金项目
国家重点研发计划项目"外来病虫害高效检测关键技术与装备研发"(2021YFD1400100,2021YFD1400103) （2021YFD1400100,2021YFD1400103）
大连海关科研项目"检疫性病原菌白蜡鞘孢菌快速检测技术的研究与应用"(2024HK05) （2024HK05）
