亚太传统医药2026，Vol.22Issue(3)：25-29,5.DOI:10.11954/ytctyy.202603005
UPLC-MS/MS法同时测定金胆片中3种胆汁酸含量
Simultaneous Determination of Three Bile Aids in Jindan Tablets by UPLC-MS/MS Methods
摘要
Abstract
Objective:To establish a rapid and accurate ultra-performance liquid chromatography-tandem mass spec-trometry(UPLC-MS/MS)method for the simultaneous determination of three bile acids in Jindan Tablets.Methods:Jindan Tablets was extracted with methanol.The chromatographic column was ACQUITY UPLC®BEHC18 column(100×2.1mm,1.7μm).Methanol-water(0.1%formic acid+5 mmol/L ammonium acetate was used as mobile phase for gradient elution.Detection was performed using an electrospray ionization source in negative ion mode with multiple reaction monitoring(MRM).Results:The peaks area of THDCA,GHDCA,and TCDCA had good linear rela-tionship with the quantity contents in the ranges of 0.103 3～51.650 0μg/mL,0.102 5～51.250 0μg/mL and 0.103 1～51.550 0μg/mL(r=0.9999,0.9995,0.9999),respectively.RSD of precision,reproducibility,stability tests and re-coveries rates were lower than 3.75%.The total content of THDCA,GHDCA,and TCDCA in 84 batches of Jindan Tablets from seven manufacturers ranged from 2.088 0 to 6.921 6 mg/g.Based on the mean total content,the manu-facturers were ranked as follows:B>A>E>C>G>F>D.Conclusion:The established method in this study is sim-ple,quick and accurate which is suitable for the rapid detection and quality evaluation of bile acids in Jindan Tablets.关键词
超高效液相色谱-串联质谱法/胆汁酸/金胆片Key words
UPLC-MS/MS Method/Bile Acid/Jindan Tablets分类
医药卫生引用本文复制引用
王源潮,王天宇,旦增次旦,魏静,向虹蕾,贾巴将芬,罗霄,李文兵,达娃卓玛..UPLC-MS/MS法同时测定金胆片中3种胆汁酸含量[J].亚太传统医药,2026,22(3):25-29,5.基金项目
国家重点研发计划-中医药现代化专项(2023YFC3504101) （2023YFC3504101）
四川省药品监督管理局项目(N5100012024000571) （N5100012024000571）
