应用化学2026，Vol.43Issue(2)：195-207,中插1-中插12,25.DOI:10.19894/j.issn.1000-0518.250378
氢键有机框架纳米酶的制备及其靶向抗菌应用
Synthesis of Hydrogen-Bonded Organic Framework Nanoenzyme for Targeting Antibacterial Applications
摘要
Abstract
Bacterial infection poses a significant hazard to public health and life.Antibiotic therapy can readily result in multidrug resistance.Despite significant efforts to enhance the effectiveness of current antibiotics or create new ones,bacteria's innate capacity to develop resistance has outpaced the rate of antibiotic development.This suggests that attempts to create sustainable antimicrobial treatments are ultimately destined to be unsuccessful.Herein,a selective therapeutic HOFzyme(Pro@HOF@apt-Cu)based on DNA-templated ultrasmall CuNPs grafted on peroxidase-mimicking FeTCPP-based HOF targeting lipopolysaccharide(LPS)is presented.FeTCPP-based HOF exhibit excellent POD-like activity,being not only able to catalyze the generation of more toxic hydroxyl radicals from H2 O2 in the infection-rich microenvironment to disrupt the bacterial periplasm,but also able to oxidize surface Cu(0)to Cu(Ⅰ),thus boosting the bioorthogonal catalytic activity.The conjugation of the HOF with aptamer allows for active targeting of LPS on the surface of gram-negative bacteria.The antibacterial action of these HOFzymes is observably more significant than non-targeted treatment.Moreover,the inherent pores of HOF facilitated the loading of prodrugs,which realized effective drug delivery and in situ synthesis,greatly reduced off-target toxicity and enhanced therapeutic effects.Overall,this synergistic therapeutic strategy shows promising potential as a future treatment for infections caused by gram-negative bacteria.关键词
氢键有机框架/生物正交/纳米酶/抗菌应用Key words
Hydrogen-bonded organic framework/Bioorthogonal/Nanozyme/Antibacterial applications分类
化学化工引用本文复制引用
陈礼,赵传奇..氢键有机框架纳米酶的制备及其靶向抗菌应用[J].应用化学,2026,43(2):195-207,中插1-中插12,25.基金项目
吉林省自然科学基金(No.20210101130JC)、国家自然科学基金(Nos.22377120,22122704,22437006,22237006,T2495262)和国家重点研发计划(Nos.2022YFA1205804,2019YFA0709202,2021YFF1200700)资助 Supported by Jilin Provincial Natural Science Foundation(No.20210101130JC),the National Nature Science Foundation of China(Nos.22377120,22122704,22437006,22237006,T2495262)and the National Key R&D Program of China(Nos.2022YFA1205804,2019YFA0709202,2021YFF1200700) （No.20210101130JC）
