中国草地学报2026，Vol.48Issue(2)：28-44,17.DOI:10.16742/j.zgcdxb.20250142
基于转录组整合分析挖掘蒺藜苜蓿非生物胁迫表观遗传调控基因
Integrated Transcriptome Analysis Reveals Epigenetic Regulatory Genes under Abiotic Stress in Medicago truncatula
摘要
Abstract
To further explore the epigenetic regulatory mechanisms underlying Medicago truncatula's response to abiotic stress,this study integrated transcriptome datasets related to salt,drought,low-temperature,and high-temperature stresses from the SRA database.Using WGCNA(weighted gene co-expression network analysis),GSEA(gene set enrichment analysis),and GO(gene ontology)enrichment analysis,we systematically identified epigenetic regulatory genes and their associated biological processes involved in abiotic stresses responses(salt,drought,low temperature,and high temperature).The results showed that 27,234,and 8 core genes related to epigenetic regulation were screened through the three ana-lytical methods,respectively.WGCNA indicated that abiotic stress-responsive genes in M.truncatula were distributed across multiple modules,with 8,5,and 5 core genes in key epigenetic-related modules participating in histone acetylation,methylation,and deacetylation,respectively.The GSEA results indi-cated that the histone methylation and histone acetylation processes constitute the most prominent regula-tory networks under drought and low-temperature stress treatments,respectively.Both histone methyla-tion and histone acetylation constituted the largest regulatory network under high-temperature stress treat-ment,while DNA methylation or demethylation was the largest regulatory network under salt stress treat-ment.From the differentially expressed genes under the four abiotic stresses(low temperature,high tem-perature,salt,and drought),GO enrichment analysis identified 30,76,77,and 88 genes enriched in 42,48,52,and 49 biological processes related to epigenetic regulation,respectively.By integrating the core genes identified through the three analytical approaches,MtrunA17Chr4g0056011 was determined as a common core gene and proposed as a candidate gene for molecular breeding of stress-resistant leguminous forage crops.关键词
蒺藜苜蓿/转录组/非生物胁迫/表观遗传/WGCNA/富集分析Key words
Medicago truncatula/Transcriptome/Abiotic stress/Epigenetic/WGCNA/Enrichment analysis分类
农业科技引用本文复制引用
赵媛媛,蒲丹,刘巧玲,杜璐多,张俊超..基于转录组整合分析挖掘蒺藜苜蓿非生物胁迫表观遗传调控基因[J].中国草地学报,2026,48(2):28-44,17.基金项目
省部共建三江源生态与高原农牧业国家重点实验室自主课题(2024-ZZ-16) （2024-ZZ-16）
青海大学青年科研基金项目(2023-QNY-6) （2023-QNY-6）
青海省"昆仑英才·高端创新创业人才—拔尖人才" （）
国家自然科学基金项目(32201452) （32201452）
