中国南方果树2025，Vol.54Issue(z1)：10-13,4.DOI:10.13938/j.issn.1007-1431.20250260
离体柠檬茎段热处理抑制黄脉病病毒效果研究简报
Preliminary report on the study of the inhibitory effect of heat treatment on yellow vein clearing virus in isolated lemon stem segments
摘要
Abstract
Heat treatment is an important method in the research of virus elimination,resistance and tolerance of lemon.In vitro culture can overcome environmental influences,shorten the breeding cycle and improve the propagation coefficient.Experiments on the temperature and duration of heat treatment were carried out on the stem segments of Eureka lemon under in vitro condi-tions,and citrus yellow vein clearing virus(CYVCV),the most harmful virus to Eureka lemon,was taken as the research object to detect the virus-inhibiting effect of heat treatment by real-time quantitative PCR.The results showed that with the increase of heat treatment temperature,the tissue structure of in vitro stem segments was damaged to varying degrees and the electrical conductiv-ity was increased.The in vitro stem segments treated at 51℃ for 30 min could develop normally with the viral load significantly inhibited,which was the extreme tolerance condition of heat treatment for in vitro Eureka lemon stem segments.关键词
柠檬/离体培养/热处理/病毒Key words
lemon/in vitro culture/heat treatment/virus引用本文复制引用
蒋雨含,晏重西,李勋兰,彭芳芳,韩国辉,魏召新..离体柠檬茎段热处理抑制黄脉病病毒效果研究简报[J].中国南方果树,2025,54(z1):10-13,4.基金项目
重庆市农业科学院绩效激励项目(cqaas2021jxj107) （cqaas2021jxj107）
重庆市技术创新与应用发展专项(CSTB2023TIAD-KPX0044)资助. （CSTB2023TIAD-KPX0044）
