中国猪业2026，Vol.21Issue(1)：3-11,9.DOI:10.16174/j.issn.1673-4645.2026.01.001
淮南猪PDK4基因克隆、生物信息学分析及组织表达研究
Cloning,bioinformatics and tissue expression profiling of the PDK4 gene in Huainan pigs
摘要
Abstract
This study aimed to clone the Pyruvate dehydrogenase kinase 4(PDK4)gene from Huainan pigs and analyze its bioinformatics characteristics,thereby providing a theoretical basis for subsequent in-depth functional verification of this gene.Using cDNA from the longissimus dorsi muscle of Huainan pigs as a template,the complete CDS region of the PDK4 gene was cloned by PCR,followed by bioinformatics prediction and analysis.The results showed that the full-length CDS of the Huainan pig PDK4 gene was 1 224 bp,encoding a polypeptide of 407 amino acids,which shared high homology with cattle,sheep,and humans.The encoded protein had a molecular weight of 46.17 kDa and was classified as an unstable protein.It contained one Adom3 domain of branched-chain alpha-keto acid dehydrogenase kinase and one C-terminal domain of histidine kinase-like ATPase,and may have strong correlations with proteins such as UCP3 and DKAT.The qRT-PCR results revealed that the PDK4 gene was widely expressed in various tissues of Huainan pigs,with relatively high expression in adipose tissue.This study provided a foundation for further investigating the biological functions of the PDK4 gene.关键词
淮南猪/背最长肌/丙酮酸脱氢酶激酶4/生物信息学/组织表达分析Key words
Huainan pig/longissimus dorsi/PDK4/Bioinformatics/tissue expression analysis分类
农业科技引用本文复制引用
郭亚苹,王璟,邢宝松,聂治国,赵雅雪,张寒冰,陈俊峰,张家庆,任巧玲,张华,杨丽玉..淮南猪PDK4基因克隆、生物信息学分析及组织表达研究[J].中国猪业,2026,21(1):3-11,9.基金项目
国家重点研发计划项目(2021YFD1301200) （2021YFD1301200）
河南省重点研发专项项目(251111110200) （251111110200）
河南省农业科学院博士科研启动费项目(2026BX55) （2026BX55）
河南省农业科学院自主创新项目(2026ZC74) （2026ZC74）
中央引导地方科技发展资金项目(Z20241471127) （Z20241471127）
