中国中西医结合杂志2026，Vol.46Issue(2)：175-183,9.DOI:10.7661/j.cjim.20250428.264
荣筋拈痛方治疗膝骨关节炎作用机制
Mechanism of Rongjin Niantong Formula in the Treatment of Knee Osteoarthritis
摘要
Abstract
Objective To study the mechanism of Rongjin Niantong Formula(RJNTF)in treating knee osteoarthritis(KOA)by regulating the glucose metabolism of rat chondrocytes through the miR-140-Sirt1/AMPK signaling axis.Methods Experiment 1:Chondrocytes were divided into control group(blank serum),interleukin-1 β(IL-1β)group(IL-1β+blank serum),and RJNTF group(IL-1β+RJNTF containing serum).IL-1β intervention lasted for 24 h,while blank serum and RJNTF containing serum intervened for 48 h.Using mitochondrial membrane potential probe(JC-1)to detect changes in mitochondrial membrane potential.Enzyme linked immunosorbent assay(ELISA)was used to detect the content of adenosine triphosphate(ATP)in cells.Real-time fluorescent quantitative nucleic acid amplification detection system(RT-qPCR)were used to detect miR-140-3p,Sirt1,gene expressions of peroxisome proliferator activated receptor gamma co-activator factor-1 alpha(PGC-1 alpha),hexokinase 2(HK2),and lactate dehydrogenase A(LDHA).Western Blot was used to detect the expressions of Sirt1,phosphorylated adenosine monophosphate activated protein kinase(p-AMPK),AMPK,PGC-1α,HK2,and LDHA proteins.Experiment 2:Chondrocytes were divided into Group A(blank serum+negative control virus),Group B(blank serum+IL-1β+negative control virus),Group C(RJNTF+IL-1β+negative control virus),Group D(blank serum+miR-140-3p knockdown lentivirus),Group E(blank serum+IL-1β+miR-140-3p knockdown lentivirus),and Group F(RJNTF drug containing serum+IL-1β+miR-140-3p knockdown lentivirus),with the same intervention time for IL-1β and serum.Using ELISA method to detect ATP content in cells.RT-qPCR was used to detect the expressions of miR-140-3p,Sirt1,PGC-1α,HK2,and LDHA genes in cells.Western Blot was used to detect the expressions of Sirt1,p-AMPK,AMPK,PGC-1α,HK2,and LDHA proteins in cells.Results Experiment 1:Compared with the control group,the IL-1β group showed a decrease in mitochondrial membrane potential and ATP content(P＜0.05),miR-140-3p gene expression and p-AMPK protein expression(P＜0.05),Sirt1 and PGC-1 α gene and protein expressions(P＜0.05),and an increase in HK2 and LDHA gene and protein expressions(P＜0.05).Compared with the IL-1β group,the RJNTF group showed an increase in mitochondrial membrane potential and ATP content(P＜0.05),miR-140-3p gene expression and p-AMPK protein expression(P＜0.05),Sirt1 and PGC-1 α gene and protein expressions(P＜0.05),and a decrease in HK2 and LDHA gene and protein expressions(P＜0.05).Experiment 2:Compared with Group A,Group B showed a decrease in ATP content(P＜0.05),a decrease in p-AMPK protein expression(P＜0.05),a decrease in Sirt1 and PGC-1 α gene and protein expressions(P＜0.05),and an increase in HK2 and LDHA gene and protein expressions(P＜0.05).Compared with group B,group C showed an increase in ATP content(P＜0.05),an increase in p-AMPK protein expression(P＜0.05),an increase in Sirt1 and PGC-1α gene and protein expressions(P＜0.05),and a decrease in HK2 and LDHA gene and protein expressions(P＜0.05).Compared with group C,group F showed a decrease in ATP content and a decrease in p-AMPK protein expression(P＜0.05),a decrease in Sirt1 and PGC-1 α gene and protein expressions(P＜0.05),and an increase in HK2 and LDHA gene and protein expressions(P＜0.05).Compared with Group E,the expression trends of various indicators in Group D were consistent with those in Group A and Group B.Compared with Group F,the expression trends of various indicators in Group E were consistent with those in Group B and Group C.Conclusion RJNTF treated KOA possibly by up-regulating miR-140-3p,Sirt1,p-AMPK,PGC-1 α expressions,and down-regulating HK2 and LDHA expressions,and regulating chondrocyte glucose metabolism.关键词
荣筋拈痛方/膝骨关节炎/软骨细胞/糖酵解/沉默信息调节因子1/单磷酸腺苷激活蛋白激酶信号通路/中药复方Key words
Rongjin Niantong Formula/knee osteoarthritis/chondrocyte/glycolysis/silent information regulator 1/adenosine monophosphate activatedprotein kinase signaling pathway/Chinese herbal compound引用本文复制引用
郑若曦,董淇,戴雨婷,吴广文,陈俊..荣筋拈痛方治疗膝骨关节炎作用机制[J].中国中西医结合杂志,2026,46(2):175-183,9.基金项目
国家自然科学基金资助项目(No.82405443) （No.82405443）
福建省自然科学基金面上项目(No.2022J01843) （No.2022J01843）
国家中医药管理局高水平中医药重点学科建设项目(No.zyyzdxk-2023106) （No.zyyzdxk-2023106）
福建中医药大学中医骨伤科学学科开放课题重大项目(No.XGS2023004) （No.XGS2023004）
