中药药理与临床2026，Vol.42Issue(1)：15-21,7.
小续命汤对光化学法诱导脑卒中小鼠神经炎症反应和血脑屏障损伤的作用及机制
Effects and Mechanisms of Xiaoxuming Decoction on Neuroinflammation and Blood-Brain Barrier Injury in the Mouse Model of Ischemic Stroke Induced by the Photochemical Method
摘要
Abstract
Objective:To investigate the effects and mechanisms of Xiaoxuming Decoction(小续命汤)extract(XXM)on the neuroinflammation and blood-brain barrier(BBB)injury in the mouse model of ischemic stroke.Meth-ods:A mouse model of ischemic stroke was established by the photochemical method.Mice were randomly grouped as follows:normal control,model control,XXM(75,150,and 300 mg/kg),and Ginkgo biloba extract(100 mg/kg).Neu-rological deficits were assessed via the modified Neurological Severity Score(mNSS).Muscle strength was evaluated through the grip strength test and hind-limb clasping test.Cerebral infarct volume was measured by TTC staining.Levels of interleukin-1β(IL-1β),interleukin-6(IL-6),interleukin-18(IL-18),tumor necrosis factor-α(TNF-α),monocyte chemoattractant protein-1(MCP-1),and intercellular adhesion molecule-1(ICAM-1)in the brain tissue were quantified by enzyme-linked immunosorbent assay(ELISA).Blood-brain barrier integrity was examined via Evans Blue staining.Expression levels of ionized calcium-binding adapter molecule 1(IBA1),glial fibrillary acidic protein(GFAP),OCCLU-DIN,and CLAUDIN-5 were measured by immunofluorescence.Results:Compared with the normal control group,the model control group showed increases in mNSS scores,clasping scores,cerebral infarct volume,levels of IL-1β,IL-6,IL-18,TNF-α,MCP-1,ICAM-1,Iba1,and GFAP(P<0.05 or P<0.01),Evans Blue leakage(P<0.01),while it show-cased down-regulated expression levels of OCCLUDIN and CLAUDIN-5 in the peri-infarct and infarct core regions(P<0.05 or P<0.01).Compared with the model control group,all the XXM groups exhibited reductions in mNSS scores,clasping scores,cerebral infarct volume,levels of IL-1β,IL-6,IL-18,MCP-1,and ICAM-1 in the brain tissue,and Evans Blue leakage(P<0.05 or P<0.01).XXM at 75 mg/kg and 300 mg/kg reduced the TNF-α level in the brain tissue(P<0.05).The expression level of GFAP was down-regulated in all the XXM groups(P<0.05 or P<0.01).The OC-CLUDIN expression in the infarct core region and CLAUDIN-5 expression in both peri-infarct and infarct core regions were up-regulated in all the XXM groups(P<0.05 or P<0.01).Conclusion:Xiaoxuming Decoction exerts anti-stroke effects by alleviating neuroinflammation and protecting the integrity of the blood-brain barrier.关键词
小续命汤/缺血性脑卒中/神经炎症/血脑屏障/离子钙结合适配器分子1/闭合蛋白/胶质纤维酸性蛋白/紧密连接蛋白-5Key words
Xiaoxuming Decoction/Ischemic stroke/Neuroinflammation/Blood-brain barrier/Ionized calcium-binding a-dapter molecule 1(IBA1)/OCCUDIN/Glial fibrillary acidic protein(GFAP)/CLAUDIN-5引用本文复制引用
张文芳,刘冬妮,冯琬迪,冯丹虹,徐双,赵闻乐,杜冠华,王月华..小续命汤对光化学法诱导脑卒中小鼠神经炎症反应和血脑屏障损伤的作用及机制[J].中药药理与临床,2026,42(1):15-21,7.基金项目
北京市自然科学基金面上项目资助(编号:7232299) （编号:7232299）
中国医学科学院医学与健康科技创新工程项目资助(编号:2021-I2M-1-069). （编号:2021-I2M-1-069）
