中药药理与临床2026，Vol.42Issue(1)：27-36,10.
益坤饮通过ERα-SDF-1/CXCR4轴活化内皮祖细胞修复受损的血管内皮细胞
Yikunyin Activated Endothelial Progenitor Cells via ERα-SDF-1/CXCR4 Axis to Repair Injured Vascular Endothelial Cells
摘要
Abstract
Objective:To investigate the mechanism of Yikunyin(益坤饮,YKY)in mediating the proliferation,mi-gration,and homing of endothelial progenitor cells(EPCs)through the estrogen receptor α(ERα)-stromal cell-derived factor(SDF-1)/chemokine receptor 4(CXCR4)axis to repair injured vascular endothelial cells.Methods:Rat bone marrow-derived EPCs were cultured and identified.A tumor necrosis factor α(TNF-α)-induced EPC injury model was established.Serum containing YKY of different concentrations was added,along with ERα inhibitor MPP and SDF-1/CXCR4 inhibitor AMD3100.MTT was used to detect cell proliferation,and Transwell was used to detect cell migration.The levels of NO,VEGF,Ang-1,Ang-2,and SDF-1 were measured by ELISA,and the tube formation capacity was meas-ured by the Matrigel tube formation method,while protein expressions of ERα,SDF-1,and CXCR4 were detected by Western blot.Results:Compared with the blank control group,the model control group showed a significant decrease in the proliferation and migration of EPCs,as well as in the contents of NO,VEGF,Ang-1,Ang-2,and SDF-1,the angiogen-esis ability,and the protein expressions of ERα,SDF-1,and CXCR4.Compared with the model control group,the serum groups containing 13.6 g/kg YKY of different concentrations significantly promoted the proliferation and migration of EPCs,increased the secretion of NO,VEGF,Ang-1,Ang-2,and SDF-1,and significantly upregulated the expression levels of total tube length,branch number,ERα,SDF-1,and CXCR4(P<0.01).After intervention with the inhibitors AMD3100 and MPP,compared with those in the blank control group,the proliferation and migration numbers of EPCs,the contents of NO,VEGF,Ang-1,Ang-2,and SDF-1,as well as the tube formation ability and the protein expressions of ERα,SDF-1,and CXCR4,in the MPP group and the AMD3100 group all decreased(P<0.01).Compared with the 20%serum group containing 13.6 g/kg YKY,the intervention effects of MPP+20%serum group containing YKY and the AMD3100+20%serum group containing YKY showed a decrease in the proliferation and migration numbers,as well as the contents of NO,VEGF,Ang-1,Ang-2,and SDF-1 secreted.The ability to promote vascular formation was weakened.The expression of SDF-1 and CXCR4 was downregulated in the MPP+YKY group and the AMD3100 group(P<0.01),and the expression of Erα was significantly downregulated in the MPP+YKY group.Conclusion:YKY can repair in-jured endothelial cells by regulating the proliferation,migration,and homing of EPCs through the ERα-SDF-1/CXCR4 axis.关键词
益坤饮/内皮祖细胞/雌激素受体α/基质细胞衍生因子/C-X-C趋化因子受体4Key words
Yikunyin/Endothelial progenitor cell/ERα/SDF-1/CXCR4引用本文复制引用
赵欣妍,李丽萍,詹群,陈霞,杜秋..益坤饮通过ERα-SDF-1/CXCR4轴活化内皮祖细胞修复受损的血管内皮细胞[J].中药药理与临床,2026,42(1):27-36,10.基金项目
江苏省自然科学基金青年项目(编号:BK20210035) （编号:BK20210035）
南京市中医药卫生青年人才项目(编号:ZYQ20012) （编号:ZYQ20012）
南京市 （）
中药制剂与临床药学研究医学重点实验室建设项目(编号:NJSYXZDSYS-2023). （编号:NJSYXZDSYS-2023）
