分析测试学报2026，Vol.45Issue(3)：543-550,8.DOI:10.12452/j.fxcsxb.25082301
基于分子印迹固相萃取/LC-MS/MS联用技术检测渔业环境中甲霜灵及其代谢物残留
Determination of Metalaxyl and Its Metabolite Residues in Aquaculture Environments Using Molecularly Imprinted Solid-phase Extraction Combined with LC-MS/MS
摘要
Abstract
This study synthesized a molecularly imprinted polymer(MIP)selective for metalaxyl us-ing oxadixyl as a dummy template,methacrylic acid and acrylamide as binary functional monomers,ethylene glycol dimethacrylate as a cross-linker,and azodiisobutyronitrile as an initiator via precipi-tation polymerization.The polymer was characterized by scanning electron microscopy and Fourier transform infrared spectroscopy,and its adsorption behavior was evaluated through isothermal and ki-netic experiments.Results confirmed high affinity and specificity toward metalaxyl and its metabolite,metalaxyl acid.The MIP was employed as a solid-phase extraction sorbent coupled with liquid chro-matography-tandem mass spectrometry(LC-MS/MS)for the analysis of aquaculture water and sedi-ment samples.The method demonstrated good linearity(r²>0.997)from 1 to 200 ng/mL,with aver-age recoveries of 86.1%-105%and relative standard deviations not more than 8.2%.Detection limits were 1.25,2.34 ng/L in water and 0.15,0.29 ng/g in sediment,and quantification limits were 4.16,4.81 ng/L in water and 0.38,0.60 ng/g in sediment,respectively.This method is sensitive,robust,and straightforward,which is suitable for routine monitoring of trace metalaxyl residues in aquaculture environments,supporting sustainable aquaculture practices.关键词
甲霜灵/甲霜灵酸/分子印迹固相萃取/液相色谱-串联质谱/渔业环境Key words
metalaxyl/metalaxyl acid/molecularly imprinted solid-phase extraction/LC-MS/MS/aquaculture environments分类
化学化工引用本文复制引用
李佳豪,周豪,尹怡,林嘉薇,赵城,单奇,王庆..基于分子印迹固相萃取/LC-MS/MS联用技术检测渔业环境中甲霜灵及其代谢物残留[J].分析测试学报,2026,45(3):543-550,8.基金项目
国家自然科学基金(32503192,32303012) （32503192,32303012）
广州市科技计划项目(2023A04J0091) （2023A04J0091）
中国水产科学研究院中央级公益性科研院所基本科研业务费(2023TD49) （2023TD49）
