口腔疾病防治2026，Vol.34Issue(3)：226-236,11.DOI:10.12016/j.issn.2096-1456.202550454
新北美圣草苷对牙周炎小鼠牙槽骨吸收和肠道菌群的影响
Effects of neoeriocitrin on alveolar bone loss and gut microbiota in mice with periodontitis
摘要
Abstract
Objective To investigate the inhibitory effect of neoeriocitrin(Neo)on ligature-induced experimental periodontitis in mice and evaluate its biosafety,providing experimental evidence for novel candidate drugs in periodonti-tis treatment.Methods This study has been approved by the Animal Welfare and Ethical Safety Committee.A peri-odontitis model was established in C57BL/6J mice using silk ligation.The mice were divided into control,periodontitis model,and three Neo treatment groups(5,10,20 mg/kg Neo).After 2 weeks of intervention,alveolar bone resorption was analyzed by micro-computed tomography(micro-CT);periodontal tissue pathological changes were observed via hematoxylin-eosin(HE)and Masson staining;osteoclasts were counted using tartrate-resistant acid phosphatase stain-ing;serum inflammatory factor levels[tumor necrosis factor-α(TNF-α),interleukin-6(IL-6),interleukin-1β(IL-1β),in-terleukin-10(IL-10)]were detected by enzyme-linked immunosorbent assay;gut microbiota changes were analyzed by 16S rRNA sequencing;and biosafety was assessed through HE staining of the heart,liver,spleen,lung,kidney,and colon,as well as zonula occludens-1(ZO-1)immunohistochemical staining of the colon.Results Compared with the periodontitis model group,the 10 mg/kg Neo treatment group showed significantly alleviated alveolar bone resorption,manifested as reduced cementoenamel junction to alveolar bone crest distance,increased bone mineral density,bone volume fraction,and trabecular thickness.Additionally,reduced inflammatory cell infiltration,improved collagen fiber arrangement,and a significantly decreased number of osteoclasts were observed in periodontal tissues.Serum levels of pro-inflammatory factors TNF-α and IL-6 were also reduced.Furthermore,10 mg/kg Neo intervention modulated the gut microbiota structure without causing significant multi-organ toxicity or impairing intestinal barrier function.Conclu-sion Neo can effectively mitigate the progression of experimental periodontitis by inhibiting alveolar bone resorption,reducing periodontal tissue inflammation,modulating systemic immunity,and improving gut microbiota.With good bio-safety,Neo is a promising candidate drug for the treatment of periodontitis.关键词
新北美圣草苷/牙周炎/骨吸收/炎症因子/肠道菌群/口-肠轴/天然化合物/破骨细胞Key words
neoeriocitrin/periodontitis/bone resorption/inflammatory factors/gut microbiota/oral-gut axis/natural compound/osteoclast分类
医药卫生引用本文复制引用
伍玉,袁志瑶,张杨珩,闫福华..新北美圣草苷对牙周炎小鼠牙槽骨吸收和肠道菌群的影响[J].口腔疾病防治,2026,34(3):226-236,11.基金项目
国家自然科学基金项目(82270979) （82270979）
江苏省医学重点学科建设单位基金项目(JSDW202246) This study was supported by the grants from National Natural Science Foundation of China(No.82270979)and Jiangsu Provincial Medical Key Discipline Cultivation Unit(No.JSDW202246). （JSDW202246）
