分析化学2026，Vol.54Issue(2)：183-194,12.DOI:10.19756/j.issn.0253-3820.251299
成簇规则间隔短回文重复序列及其相关蛋白技术在疾病即时检测中的应用进展
Progress of Clustered Regularly Interspaced Short Palindromic Repeats-associated Proteins Technology in Point-of-Care Testing for Disease
摘要
Abstract
Disease prevention and control have always been crucial to public health and national security.Point-of-care testing(POCT),a method that utilizes portable instruments and reagents to obtain rapid test results at the sampling site,has demonstrated significant advantages and development potential in disease prevention and control while effectively improving diagnostic efficiency.However,many of the reported POCT methods have been limited in practical use due to their low sensitivity.In recent years,the clustered regularly interspaced short palindromic repeats(CRISPR)-CRISPR associated proteins(CRISPR/Cas)system,with its powerful trans-cleavage activity,has gained prominence as an effective signal amplification strategy in detection fields.The integration of CRISPR/Cas systems with POCT has led to rapid advancements in detecting both nucleic acid and non-nucleic acid biomarkers.This paper outlined CRISPR/Cas system types,sensing principles,and target analytes,and categorized CRISPR-based POCT detection methods by signal output modes,including fluorescence,electrochemistry,solution colorimetry,test strip colorimetry,and Raman spectroscopy.Finally,it summarized current developments in this field while discussing existing challenges and future directions.关键词
成簇规则间隔短回文重复序列及其相关蛋白/电化学/荧光/比色法/试纸条比色/拉曼光谱/评述Key words
Clustered regularly interspaced short palindromic repeats-associated proteins/Electrochemistry/Fluorescence/Colorimetry/Test strip colorimetry/Raman spectroscopy/Review引用本文复制引用
王颖慧,李想,张瑞巧,曾景斌,温聪颖..成簇规则间隔短回文重复序列及其相关蛋白技术在疾病即时检测中的应用进展[J].分析化学,2026,54(2):183-194,12.基金项目
国家自然科学基金项目(No.22376218)、山东省自然科学基金项目(No.ZR2022JQ07)和中央高校基本科研业务费专项基金项目(No.25CX02013A)资助. Supported by the National Natural Science Foundation of China(No.22376218),the Natural Scientific Foundation of Shandong Province(No.ZR2022JQ07)and the Fundamental Research Funds for the Central Universities of China(No.25CX02013A). （No.22376218）
