分析化学2026，Vol.54Issue(2)：205-213,中插3-中插15,22.DOI:10.19756/j.issn.0253-3820.251260
AuPd@FexOy纳米酶标记的免疫层析试纸条用于D-二聚体的快速检测
Lateral Flow Immunoassay Based on AuPd@FexOy Nanozymes and Its Application for Rapid Detection of D-dimer
摘要
Abstract
D-dimer detection is a crucial step in diagnosis of venous thromboembolism(VTE).In this study,a typical"sandwich"lateral flow immunoassay(LFIA)was established via modification of AuPd@FexOy nanoenzyme(AuPd@FexOy)with the specific antibodies of D-dimer.The AuPd@FexOy NP-labeled LFIA(AuPd@FexOy-LFIA)was used for rapid detection of D-dimer levels in human plasma.Due to its excellent peroxidase-like(POD-like)activity,in the enhanced colorimetric mode of AuPd@FexOy catalyzing H2O2 oxidation of 3,3',5,5'-tetramethylbenzidine(TMB),the limit of detection of AuPd@FexOy-LFIA was as low as 2.4 ng/mL,which was significantly lower than the health threshold of 500 ng/mL.Furthermore,the AuPd@FexOy-LFIA had good selectivity,reproducibility,and storage stability.The D-dimer detection results of 41 clinical plasma samples were well correlated with those of commercially available enzyme-linked immunosorbent assay(ELISA)detection kits(R2=0.98).The research results indicated that the AuPd@FexOy-LFIA could be used for rapid(<15 min)and sensitive detection of D-dimer in plasma,which showed potential in clinical diagnosis.关键词
AuPd@FexOy纳米酶/侧流免疫层析/D-二聚体/血浆/静脉血栓栓塞Key words
AuPd@FexOy nanozymes/Lateral flow immunoassay/D-dimer/Plasma/Venous thrombosis embolism引用本文复制引用
吴复超,王皓冬,孙旭东,李晓桐,宋丹,佟倩,李风华,王振新..AuPd@FexOy纳米酶标记的免疫层析试纸条用于D-二聚体的快速检测[J].分析化学,2026,54(2):205-213,中插3-中插15,22.基金项目
吉林省科技厅-医药健康重点研发项目(No.20230203173SF)和吉林省医疗卫生人才专项项目(No.JLSRCZX2025-002)资助. Supported by the Jilin Provincial Science and Technology Department(No.20230203173SF)and the Jilin Provincial Special Project for Medical and Health Talents(No.JLSRCZX2025-002). （No.20230203173SF）
