分析化学2026，Vol.54Issue(2)：214-224,11.DOI:10.19756/j.issn.0253-3820.251334
基于铜-谷氨酸聚合物纳米酶的乙酰胆碱酯酶抑制剂类农药的检测研究
Detection of Acetylcholinesterase Inhibitor Pesticides Based on Copper-Glutamic Acid Polymer Nanozyme
摘要
Abstract
Copper-glutamic acid polymer nanoparticles(Cu-Glu CPNs)were prepared by one-step precipitation method from copper nitrate and glutamic acid in aqueous solution at room temperature and atmospheric pressure for detection of acetylcholinesterase(AChE)inhibitor pesticides.AChE catalyzed the hydrolysis of acetylcholine(ACh)to choline(Ch),which was oxidized by choline oxidase(ChOx)to produce hydrogen peroxide(H2O2).H2O2 was further catalyzed by Cu-Glu CPNs with peroxidase-like activity to oxidize the colorless 3,3',5,5'-tetramethylbenzidine(TMB)to a blue oxidation product,showing a distinct absorption peak at 650 nm.When organophosphorus pesticides(OPs)or carbamate pesticides(CPs)and other AChE inhibitor pesticides were present,they indirectly inhibited the oxidation of TMB,resulting in a decrease in absorbance at 650 nm.The decrease was related to the pesticide concentration,thus achieving colorimetric detection of pesticides.Under the optimal conditions,the limits of detection(LOD)for OPs pesticide malathion(Mal)and CPs pesticide carbaryl(Car)were 2.14 ng/mL and 1.29 ng/mL,respectively.This method was applied to detection of Mal and Car in apples,cucumbers,tomatoes and oranges with recovery rates ranging from 91.6%to 105.7%and 87.1%to 118.6%,respectively,indicating its potential for practical application in agricultural product testing.关键词
铜-谷氨酸聚合物/类过氧化物酶活性/乙酰胆碱酯酶/比色传感/农药检测Key words
Copper-glutamic acid polymer/Peroxidase-like activity/Acetylcholinesterase/Colorimetric sensing/Pesticide detection引用本文复制引用
郭雅辉,雷琳,岳泽嘉,李改茹,卢沙沙,孙健..基于铜-谷氨酸聚合物纳米酶的乙酰胆碱酯酶抑制剂类农药的检测研究[J].分析化学,2026,54(2):214-224,11.基金项目
新疆维吾尔自治区自然科学基金项目(No.2025D01E33)、新疆医科大学高层次人才启动项目和国家级科研创新人才培育项目(No.XYD2024GR03)资助. Supported by the Natural Science Foundation of Xinjiang Uygur Autonomous Region(No.2025D01E33),the High-level Talents Start-up Project of Xinjiang Medical University and the National Research Innovation Talents Cultivation Project(No.XYD2024GR03). （No.2025D01E33）
