福建农业学报2025，Vol.40Issue(12)：1240-1249,10.DOI:10.19303/j.issn.1008-0384.2025.12.003
酵母硒对产蛋高峰后期蛋鸡生化指标、抗氧化功能及脂质代谢的影响
Effects of Yeast Selenium on Serum Biochemical Indexes,Antioxidant Function and Lipid Metabolism of Laying Hens in Late Laying Peak Period
摘要
Abstract
[Objective]Effects of supplementing feed with selenium-containing yeast (SY) on the serum biochemical indices,antioxidative function,and lipid metabolism of hens in late stage of peak egg production were studied.[Method]Four hundred forty eight 50-week-old Hy-Line Brown egg-laying hens were randomly divided into 4 groups,each comprising 7 replicates with 16 birds per replicate. Aside from a control group of the birds fed on a basal diet containing 0.092 mg·kg−1 selenium,three treatment groups were given forage with added SY at 0.15,0.30,and 0.45 mg·kg−1 for the gradient selenium supplementation in diet of 0.221,0.368,and 0.505 mg·kg−1,respectively. After a 10-day pre-test period,a trial lasting 56 d was executed. At the end of the trial,blood and liver tissue samples were collected from the hens for measurements of antioxidant activity,serum biochemistry,and lipid metabolism.[Results]Compared with control,(1) the addition of 0.30 mg·kg−1 SY significantly increased the serum alkaline phosphatase (ALP) and reduced the uric acid (P＜0.05) of the hens;(2) all SY supplementations significantly rose the total antioxidant capacity (T-AOC) and activity of nuclear factor erythroid 2-related factor 2 (Nrf2) in serum (P＜0.05),while the 0.30 mg·kg−1 and 0.45 mg·kg−1 additions resulted in significant increases on the serum reduced glutathione (GSH-Px) activity (P＜0.05),the added 0.30 mg·kg−1 SY yielded significantly higher activity of glutathione S-transferase (GST) in serum (P＜0.05),the 0.30 mg·kg−1 and 0.45 mg·kg−1 additions provided significantly enhanced activities of GST and Nrf2 in the liver (P＜0.05),and 0.45 mg·kg−1 SY gave significant rises on GSH-Px and T-AOC activities (P＜0.05);(3) the supplementations significantly upregulated the gene expressions of glutathione peroxidase 2(GPX2) and GPX4 in the liver (P＜0.05),and when the SY addition reached 0.30–0.45 mg·kg−1,the mRNA expression of GPX1 was significantly upregulated (P＜0.05) but not those of GPX3,superoxide dismutase 1 (SOD1),and superoxide dismutase 2 (SOD2) (P＞0.05);(4) no significant differences in the serum and liver lipid metabolism indices,including the total cholesterol (T-CHO),triglycerides (TG),high-density lipoprotein cholesterol (HDL-C),and low-density lipoprotein cholesterol (LDL-C),for all treatment groups were found (P＞0.05).[Conclusion]Dietary supplementation with 0.30–0.45mg·kg−1 SY significantly increased the antioxidative defense capacity,serum biochemical indices,and expressions of certain antioxidant-related genes,thereby helping maintain the metabolic homeostasis of the hens in late peak egg-laying period.关键词
酵母硒/蛋鸡/血清生化/抗氧化/脂代谢Key words
yeast selenium/egg-laying hen/serum biochemistry/antioxidation/lipid metabolism分类
农业科技引用本文复制引用
郭智,周振乾,胡引霞,朱敬文,虎新林,秦士贞..酵母硒对产蛋高峰后期蛋鸡生化指标、抗氧化功能及脂质代谢的影响[J].福建农业学报,2025,40(12):1240-1249,10.基金项目
国家自然科学基金项目(32360848) （32360848）
甘肃省教育厅高校教师创新基金项目(2026A-061) （2026A-061）
