福建农业学报2025，Vol.40Issue(12)：1250-1257,8.DOI:10.19303/j.issn.1008-0384.2025.12.004
牡丹R2R3-MYB转录因子PoMYB96基因的克隆及对多重非生物胁迫的响应
Cloning and Characterization of PoMYB96,an R2R3-MYB Transcription Factor Gene from Tree Peony,and Its Responses to Multiple Abiotic Stresses
摘要
Abstract
[Objective]PoMYB96 related to the response to abiotic stresses of Paeonia ostii was cloned to analyze the roles it played in regulating growth of the plant.[Method]The R2R3-MYB transcription factor PoMYB96 that regulates the response of tree peony to high or low temperature,drought,and salinity stresses,which can severely hinder the normal growth of the plant,was cloned using RT-PCR. Sequence and encoded protein of the gene were analyzed. An evolutionary tree was constructed between PoMYB96 and MYB family members from Arabidopsis thaliana. Expressions of the gene in the leaves under high and low temperatures,draught,flooding,and high salinity were determined by qRT-PCR.[Result]The full-length cDNA sequence of PoMYB96 was 984bp encoding 327 amino acids. The protein contained two conserved SANT domains and shared the highest homology with AtMYB96 of A.thaliana. Its promoter region harbored anaerobic-,salicylic acid-,and light-responsive elements,as well as hormone-responsive elements for ABA,gibberellin,and jasmonic acid. The gene was expressed in the roots,stems,leaves,flowers,stamens,and pistils,with the highest level observed in the leaves. Its relative expression was significantly upregulated under low or high temperature,drought,waterlogging,and salt stress.[Conclusion]PoMYB96 participated in the response of tree peony to abiotic stresses and could be a candidate marker for molecular breeding selection.关键词
牡丹/MYB转录因子/PoMYB96/非生物胁迫/基因克隆/表达分析Key words
Paeonia ostii/MYB transcription factor/PoMYB96/abiotic stress/gene cloning/expression analysis分类
农业科技引用本文复制引用
张利,孔羚富,唐欣,范志斌,邬博稳,刘高峰,庄涵韵..牡丹R2R3-MYB转录因子PoMYB96基因的克隆及对多重非生物胁迫的响应[J].福建农业学报,2025,40(12):1250-1257,8.基金项目
菏泽学院博士基金(XY19BS16、XY16BS34) （XY19BS16、XY16BS34）
山东省高等学校青创人才引育计划建设团队项目(2021年) （2021年）
菏泽学院培育项目(XY18PY05) （XY18PY05）
菏泽学院牡丹研究院培育项目(PR15) （PR15）
山东省自然科学青年基金项目(ZR2020QC166) （ZR2020QC166）
