贵州农业科学2026，Vol.54Issue(2)：1-12,12.DOI:10.3969/j.issn.1001-3601.2026.02.001
48份鲜食葡萄种质遗传多样性的SSR分析
SSR Analysis on Genetic Diversity of 48 Table Grape Germplasm
摘要
Abstract
[Objective]The genetic diversity level and genetic relationship of 48 table grape germplasm were explored to provide a reference for identification of grape germplasm,conservation of genetic diversity and innovation of grape germplasm resources.[Method]The genetic diversity and genetic structure of 48 table grape germplasms in Yantai region were analyzed by SSR and clustering technology respectively to establish their corresponding DNA finger-print chromatograms and molecular identity cards.[Result]The Shannon's information index,Nei's gene diversity index and polymorphism information content of 9 pairs of SSR primers(A1—A4,B1—B3,C1—C2)with good polymorphism and clear bands selected from 48 table grape germplasm were 2.37,0.86 and 0.85 respectively,which indicated that there were significant genetic differences and abundant genetic diversity among 48 table grape germplasm.The DNA finger-print chromatograms and molecular identity cards of 48 table grape germplasm were successfully constructed.The clustering results showed that Autumn Royal had a significantly distant genetic relationship with the other table grape germplasm.When the genetic distance was 0.05,the other 47 grape germplasm could be divided into two major groups.The groupⅠcould be further divided into 2 subgroups(genetic distance was 0.08),and the group Ⅱ could be further divided into 11 subgroups(genetic distance was 0.10),with three levels of 0.26,0.20,and 0.30 genetic distance.The clustering results were roughly consistent with the classification clustering based on agronomic morphological characteristics and maturity season.[Conclusion]9 pairs of primers with good polymorphism are successfully screened from 48 table grape germplasm.The distinction rate of table grape germplasm reaches 100%based on SSR technology and the SSR molecular fingerprint chromatogram and molecular identification cards of 48 table grape germplasm are successfully constructed.关键词
SSR/引物/多态性/鲜食葡萄/分子身份证/聚类分析/指纹图谱Key words
SSR/primer/polymorphism/table grape/molecular identity card/clustering analysis/fingerprint chromatogram分类
农业科技引用本文复制引用
宋超然,曹朝,李俊鹏,陈晓峰..48份鲜食葡萄种质遗传多样性的SSR分析[J].贵州农业科学,2026,54(2):1-12,12.基金项目
国家重点研发计划项目"主要瓜类蔬菜重要品质关键基因挖掘和功能鉴定"(2023YFE0206900) （2023YFE0206900）
烟台市校地融合发展项目"绿色高效设施农业工程技术创新中心"(2023XDRHXMPT12) （2023XDRHXMPT12）
中国农业大学本科生科研训练计划(URP)项目"STR分子标记分析烟台地区鲜食葡萄种质资源遗传多样性"(U2024005) （URP）
