华南农业大学学报2026，Vol.47Issue(2)：216-224,9.DOI:10.7671/j.issn.1001-411X.202508004
水稻黄叶黄颖性状的遗传分析及应用
Genetic analysis and application of the traits of yellow leaf and yellow glume in rice
摘要
Abstract
[Objective]To screen and apply sterile lines with phenotypic marker traits,and enhance the seed production purity in two-line hybrid rice.[Method]In this study,the yellow-green leaf trait(YSL)obtained through natural mutation was introduced into two-line sterile line of rice(YOS-1),resulting in a new two-line sterile line(YOS-2)carrying the yellow-green leaf marker.Combined with phenotype observation and data calculation of leaf color,determination of chlorophyll content,genetic analysis,transcriptome analysis,and candidate gene screening,the regulatory mechanism of the target genes on rice leaf color was clarified.The basic agronomic traits of YOS-2 were compared with its counterpart YOS-1.Furthermore,using each as the female parent,ten hybrid combinations were respectively constructed,and F1 generations were subjected to comparative performance analysis.[Result]In the F3 population of YSL×R1173,the contents of chlorophyll a and chlorophyll b in the seedling and tillering stages of the yellow leaf and yellow glume materials were extremely significantly lower than those of the green leaf and green glume materials ⩽(P 0.01).The phenotypic characteristics of yellow leaf and yellow glume materials as well as green leaf and green glume materials were stable at each development stage.The yellow leaf and yellow glume traits were controlled by a pair of recessive nuclear genes.Compared to YOS-1,the three agronomic traits of YOS-2(plant height,flag leaf length and flag leaf width)were predominantly governed by additive effects.Additionally,the average yield of hybrid combinations derived from YOS-2 was slightly higher than that of combinations derived from YOS-1.In the application of hybrid rice,YOS-2 could completely replace YOS-1.Three candidate genes(LOC_Os08g06220,LOC_Os08g06280,and LOC_Os08g07720)associated with the yellow leaf and yellow glume traits were initially screened out via BSA sequencing,transcriptome data analysis and gene annotation information in the molecular breeding database.Subcellular localization prediction analysis indicated that LOC_Os08g06220 was localized in chloroplast,LOC_Os08g06280 in cell membrane,and LOC_Os08g07720 in cytoplasm.[Conclusion]The breeding of YOS-2(a male sterile line with yellow leaves and yellow glumes)has further enriched the germplasm pool of leaf-color marker-based male sterile lines,and simultaneously promoted the application of phenotypic marker traits in hybrid rice production.The exploration of genes controlling yellow leaf and yellow glume traits is conducive to further understanding the regulatory mechanisms of rice photosynthesis,chlorophyll synthesis and degradation,as well as growth and development.关键词
水稻/两系不育系/叶色标记/BSA/转录组Key words
Rice/Two-line sterile line/Leaf color marker/BSA/Transcriptome分类
农业科技引用本文复制引用
梁欣,李烊烊,周丹华,陈淳,胡朝旭,王加峰,郭涛,黄明..水稻黄叶黄颖性状的遗传分析及应用[J].华南农业大学学报,2026,47(2):216-224,9.基金项目
国家重点研发计划(2022YFD1200703) （2022YFD1200703）
广州市重点研发计划(202206010015) （202206010015）
