黑龙江畜牧兽医Issue(3)：9-14,6.DOI:10.13881/j.cnki.hljxmsy.2024.12.0038
百里香精油对能量负平衡奶牛免疫功能和氧化应激的影响
Effects of thyme essential oil on immune function and oxidative stress in dairy cows with negative energy balance
摘要
Abstract
To investigate the effects of thyme essential oil on immune function and oxidative stress in dairy cows with negative energy balance(NEB),25 perinatal Holstein cows with a body weight of(425±25)kg were selected.Among them,5 cows in positive energy balance(PEB)were assigned as the PEB control group;the remaining 20 NEB cows were randomly divided into four groups,namely the NEB control group,low-dose thyme essential oil group(Thyme-L),medium-dose thyme essential oil group(Thyme-M),and high-dose thyme essential oil group(Thyme-H),with 5 cows in each group.Cows in the Thyme-L group were gavaged with 10.9 mL of thyme essential oil plus 10.9 mL of normal saline per head per day.Cows in the Thyme-M group were gavaged with 16.35 mL of thyme essential oil combined with 5.45 mL of normal saline per head per day.The Thyme-H group was gavaged with 21.8 mL of thyme essential oil alone per head per day.Both the PEB control group and NEB control group were gavaged with 21.8 mL of normal saline per head per day,and the gavage period lasted for 14 days.Blood samples were collected on days 0(before gavage),7,14,and 21,and reagent kits were used to determine serum levels of immune factors(IL-1β,IL-2,IL-6,IL-21,IL-4,MIP-1,IP-10,IgA,IgG and IgM)and oxidative stress indicators(T-AOC,GSH-Px,SOD,CAT,and MDA)in each group.The results showed that during the experiment,compared with the PEB control group,the serum levels of IL-1β,IL-2,IL-6,IL-21,IP-10,MIP-1,and MDA in the NEB control group were extremely significantly increased(P<0.01),while the levels of IL-4,IgM,IgA,IgG,GSH-Px,CAT,SOD,and T-AOC were extremely significantly decreased(P<0.01).With the extension of gavage time,the serum levels of IL-1β,IL-2,IL-6,IL-21,IP-10,MIP-1,and MDA in the Thyme-L,Thyme-M,and Thyme-H groups showed an overall decreasing trend,while the levels of IL-4,IgM,IgA,IgG,GSH-Px,CAT,SOD,and T-AOC presented an overall increasing trend.Specifically,with the increase in thyme essential oil dose,the levels of IL-1β,IL-2,IL-6,IL-21,IP-10,MIP-1,and MDA decreased gradually on days 7,14,and 21,whereas the levels of IL-4,IgM,IgA,IgG,GSH-Px,CAT,SOD,and T-AOC increased gradually.On day 21,there was no significant difference in the serum levels of IL-2,IL-21,IL-4,IP-10,IgM,IgG,CAT,SOD,and T-AOC between the Thyme-H group and the PEB control group(P>0.05).Consistently,on day 21,the levels of IL-1β,IL-2,IL-6,IL-21,IP-10,MIP-1,and MDA in the Thyme-L,Thyme-M,and Thyme-H groups were extremely significantly lower than those in the NEB control group(P<0.01),while the levels of IL-4,IgG,CAT,GSH-Px,SOD,and T-AOC were extremely significantly higher than those in the NEB control group(P<0.01).These results suggested that gavage administration of thyme essential oil could improve the immune function and alleviate the oxidative stress response of dairy cows with negative energy balance.关键词
百里香精油/能量负平衡/围产期/奶牛/免疫功能/氧化应激Key words
thyme essential oil/negative balance of energy/perinatal period/dairy cow/immune function/oxidative stress分类
农业科技引用本文复制引用
段莹莹,孙苗苗,孙旭东,徐闯..百里香精油对能量负平衡奶牛免疫功能和氧化应激的影响[J].黑龙江畜牧兽医,2026,(3):9-14,6.基金项目
国家重点研发计划青年科学家项目(2023YFD1802100) （2023YFD1802100）
