黑龙江畜牧兽医Issue(3):15-22,8.DOI:10.13881/j.cnki.hljxmsy.2025.09.0006
日粮中添加不同剂量氯化胆碱对断奶湖羊发情时间及生殖激素变化的影响
Effects of different doses of choline chloride in diet on estrus onset and reproductive hormone changes in weaned Hu sheep
摘要
Abstract
In order to explore the effects of coated choline chloride on the onset of estrus in weaned Hu sheep,48 weaned Hu sheep were randomly divided into 4 groups,with 12 sheep in each group.The control group was fed with the basal diet,and the experimental groups were supplemented with 1 g/sheep(experimental group Ⅰ),3 g/sheep(experimental group Ⅱ),and 5 g/sheep(experimental group Ⅲ)of coated choline chloride in the basal diet,respectively.The pre-test period was 8 days and the formal period was 22 days.During the test,the initial weight,final weight,and the number of sheep in estrus were counted.The weight gain,average daily weight gain,and estrus rate of sheep were calculated.Estradiol(E2),progesterone(P4),luteinizing hormone(LH),and follicle stimulating hormone(FSH)level were measured by enzyme-linked immunosorbent assay(ELISA).The results showed that the final weight,weight gain,and average daily gain in experimental group Ⅱ were significantly higher than those in the control group(P<0.05).The estrus rate was higher than that of the control group,and the estrus occurred 8 days earlier than that of the control group.The LH level in the experimental group Ⅱ was significantly higher than that in the control group on the days 7 and 16(P<0.05),and was higher on days 1,3,11,17,and 22,but the difference was not significant(P>0.05).The FSH level was significantly higher than that in the control group on days 7,9,11,13,18,and 22(P<0.05),while it was higher on days 15,16,17,and 21,but the differences were not significant(P>0.05).The level of E2 was significantly higher than that in the control group on days 3,16,17,18,and 19(P<0.05),while it was higher on days 1,5,7,9,11,and 15,but the differences were not significant(P>0.05).The level of P4 was significantly higher than that in the control group on days 5,9,11,and 17(P<0.05),while it was higher on days 1,7,13,15,16,18,and 22,but the differences were not significant(P>0.05).The hormone levels in group Ⅲ were significantly better than those in the control group at some time periods(P<0.05),but there was no significant difference between experimental group Ⅲ and group Ⅱ(P>0.05),and its daily gain was significantly lower than that in group Ⅱ(P<0.05).It was indicated that the addition of 3 g/sheep coated choline chloride in the conventional basal diet could effectively promote the growth and weight gain of weaned Hu sheep and advance the onset of estrus,which could provide an effective technical scheme for regulating the onset of estrus in weaned Hu sheep.关键词
氯化胆碱/断奶湖羊/初情期/生殖激素/日增重Key words
choline chloride/weaned Hu sheep/first estrus/reproductive hormones/daily gain分类
农业科技引用本文复制引用
吴晛,赵宗胜,武兴龙,南颖,康佳佳,李明山,孙浩宇,杨圣贤..日粮中添加不同剂量氯化胆碱对断奶湖羊发情时间及生殖激素变化的影响[J].黑龙江畜牧兽医,2026,(3):15-22,8.基金项目
国家自然科学基金项目(31260770) (31260770)
兵团农业核心技术攻关项目"多胎萨福克羊新品种培育及应用示范项目"(兵团农业GG项目) (兵团农业GG项目)