黑龙江畜牧兽医Issue(3)：23-30,8.DOI:10.13881/j.cnki.hljxmsy.2025.07.0104
马胃蝇幼虫鉴定方法研究进展
Research progress on identification methods of Gasterophilus larvae
摘要
Abstract
Gastrogasteriasis is a severe parasitic disease caused by larvae of the genus Gasterophilus in the family Oestridae parasitizing the gastrointestinal mucosa of equids.In China,the main dominant species causing the disease include Gasterophilus pecorum,Gasterophilus intestinalis,Gasterophilus nasalis,Gasterophilus haemorrhoidalis,Gasterophilus nigricornis and Gasterophilus inermis.As a major horse breeding province,Inner Mongolia Autonomous Region is seriously affected by Gastrogasteriasis disease,which has caused significant economic losses to the horse industry.At present,pathogen identification mainly depends on morphological,molecular biological and serological diagnostic methods.Morphological identification is based on the observation of larval body color and body surface spine arrangement pattern at the macro level,while the micro level focuses on the subtle differences between species with specific ultrastructure.Molecular biology techniques could effectively distinguish the other four species except G.haemorrhoidalis and G.inermis;in serological diagnosis,an indirect ELISA detection method has been established based on the crude antigen and salivary gland antigen of the three-stage Gasterophilus larvae.This paper reviewed the relevant research progress at home and abroad,and summarized the life history and morphological characteristics of the above six dominant species,aiming to provide a reference for the identification of the third-stage larvae of Gasterophilus.关键词
马胃蝇/三期幼虫/形态学/分子生物学/血清学/研究进展Key words
Gasterophilus/the third-stage larvae/morphology/molecular biology/serology/research progress分类
农业科技引用本文复制引用
孙一鸣,王腾宇,谢铭铭,陆静,左路超,王文龙..马胃蝇幼虫鉴定方法研究进展[J].黑龙江畜牧兽医,2026,(3):23-30,8.基金项目
国家自然科学基金项目(32360884) （32360884）
内蒙古自治区直属高校基本科研业务费项目(BR231406) （BR231406）
内蒙古一流学科科研专项(YLXKZX-NND-012) （YLXKZX-NND-012）
