基础医学与临床2026，Vol.46Issue(3)：311-316,6.DOI:10.16352/j.issn.1001-6325.2026.03.0311
淫羊藿苷通过抑制PI3K-AKT-血管增生治疗模型小鼠银屑病
Icariin treats for psoriasis of mouse models through inhibition of PI3K-AKT-vascular proliferation pathway
摘要
Abstract
Objective To explore the mechanism of icariin(ICA)in the treatment of psoriasis.Methods Thirty-six C57BL/6 mice were randomly divided into 6 groups with 6 in each:a blank(Ctrl)group,a model group,a positive control group treated with methotrexate,and experimental groups treated respectively with low,medium,and high doses of ICA.Ann imiquimod(IMQ)-induced psoriasis animal model was established.Methotrexate was given by intragastric administration at a concentration of 1 mg/kg.The animals in low,medium,and high dose ICA groups received intragastric administration of 0.4 mg/100 mL,0.8 g/100 mL and 1.2 g/100 mL respec-tively.On day 6 of the experiment,dorsal skin samples were collected from all six groups of mice for Hematoxy-lin-Eosin(HE)staining microscopy.RT-qPCR was used to measure the inflammatory level of Il-6,Il-17a and Tnf-α in the mouse skin.Immunofluorescence double staining was employed to observe CD31 and VEGF level in the mouse skin.Western blot was used to detect the protein level of PI3K,AKT,p-PI3K,and p-AKT in the mouse skin.Results Compared with the model group,the high-dose ICA group significantly improved IMQ-in-duced skin thickening(P＜0.000 1)and reduced the PASI score in mice.The high-dose ICA group significantly decreased mRNA level of Tnf-α,Il-6 and Il-17a in the skin of psoriasis mice(P＜0.000 1)and reduced the lev-el of CD31 and VEGF in the skin of psoriasis mice.Additionally,the high-dose ICA group reduced protein level of PI3K(P＜0.05),AKT(P＜0.05),p-PI3K(P＜0.05)and p-AKT(P＜0.01)in the mouse skin.Conclusions High-dose 1.2 g/100 mL ICA exhibits superior anti-psoriatic properties,with its mechanism poten-tially involving inhibition of PI3K-AKT-mediated angiogenesis.关键词
淫羊藿苷/银屑病/PI3K-AKT信号通路/血管增生Key words
icariin/psoriasis/PI3K-AKT signaling pathway/angiogenesis分类
医药卫生引用本文复制引用
林清,杨斌,朱榕嘉,赵春华,宋坪..淫羊藿苷通过抑制PI3K-AKT-血管增生治疗模型小鼠银屑病[J].基础医学与临床,2026,46(3):311-316,6.基金项目
北京市高层次创新创业人才支持计划"登峰、青苗、春蕾"项目(G202514020) （G202514020）
国家自然科学基金(82300129,82474345) （82300129,82474345）
中国医学科学院医学与健康科技创新工程(2022-I2M-1-012) （2022-I2M-1-012）
高等学校学科创新引智计划(B18007) （B18007）
全国重点实验室经费(2060204) （2060204）
