基础医学与临床2026，Vol.46Issue(3)：332-338,7.DOI:10.16352/j.issn.1001-6325.2026.03.0332
血浆IgG N-糖肽绝对定量方法用于筛选早期慢性阻塞性肺疾病诊断标志物
Absolute quantification of plasma IgG N-glycopeptides as a biomarker for diagnosis of the early COPD
摘要
Abstract
Objective To investigate the potential of the absolute quantifaction of plasma IgG N-glycopeptides for the early diagnosis of chronic obstructive pulmonary disease(COPD).Methods Research objects were randomly selected from the healthy control(HC)group,the early COPD(ECOPD)group and the stable COPD(COPD)group from previous research cohort(20 cases for each group),and targeted differential glycopeptides were selected based on the previously obtained relative quantitative data of plasma IgG N-glycopeptides.Absolute concentration of glycopeptide was measured with internal standard method.The linear relationship between the absolute concentra-tion of the targeted glycopeptides and the ratio of forced expiratory volume in one second to forced vital capacity(FEV1/FVC)was analyzed.The receiver operating characteristic(ROC)curves were generated to compare the di-agnostic efficacy of individual glycopeptides indicators,age,smoking index and their combination.Results The relative quantification showed that the plasma level of IgG2 G2FS glycopeptides was significantly higher in the HC group than that in the ECOPD and COPD groups(P＜0.05).The absolute quantification further clarified a suc-cessive decrease in its concentration across the HC,ECOPD,and COPD groups,with a particular difference be-tween the HC and COPD groups(P＜0.001).ROC analysis indicated that the diagnostic efficacy of the plasma IgG2 G2FS concentration for ECOPD(area under curve,AUC=0.765)was superior to that of age(AUC=0.624)or smoking index(AUC=0.602).However,the combined model incorporating IgG2 G2FS,age,and smoking in-dex(AUC=0.780)showed comparable performance to IgG2 G2FS alone.Conclusions The absolute concentration of plasma IgG2 G2FS exhibits competitive diagnostic value for early COPD,suggesting plasma IgG2 G2FS is a po-tential biomarker for early digestion of COPD.关键词
慢性阻塞性肺病/早期诊断/N-糖基化修饰/免疫球蛋白/绝对定量Key words
chronic obstructive pulmonary disease/early diagnosis/N-glycosylation/immunoglobulin/absolute quantification分类
生物科学引用本文复制引用
刘肇一,尹美玲,王琳,周巾煜..血浆IgG N-糖肽绝对定量方法用于筛选早期慢性阻塞性肺疾病诊断标志物[J].基础医学与临床,2026,46(3):332-338,7.基金项目
国家自然科学基金(22574174) （22574174）
