基础医学与临床2026，Vol.46Issue(3)：352-358,7.DOI:10.16352/j.issn.1001-6325.2026.03.0352
敲降MALAT1减轻ox-LDL诱导的巨噬细胞炎性反应和细胞损伤
Knock-down MALAT1 reduces ox-LDL-induced-inflammation and cell damage of macrophages
摘要
Abstract
Objective To explore the role and potential mechanism of metastasis-associated lung adenocarcinoma transcript 1(MALAT1)in inflammatory response and cell damage of macrophages induced by oxidized low density lipoprotein(ox-LDL).Methods THP-1 cells were induced to differentiate into macrophages by phorbol ester(PMA).Macrophages were co-cultured with ox-LDL to construct a cell injury model.Lipid accumulation was ob-served by Oil Red O staining microscopy.MALAT1 was knocked down by transfection of shRNA.The experimental group included control group,ox-LDL group,ox-LDL+shNC group and ox-LDL+shMALAT1 group.Total cholesterol and triglyceride in cells were measured.Apoptosis was detected by flow cytometry.The level of IL-6,TNFα and MCP-1 were detected by ELISA.The expression of NLRP3,RIPK1,caspase-8,p65 and SR-A was detected by RT-qPCR and Western blot.Results Compared with the control group,the cell lipid accumulation,total cholesterol and triglyceride contents,cell apoptosis,IL-6,TNFα and MCP-1 levels and the expression of NLRP3,RIPK1,caspase-8,p-p65 and SR-A increased significantly in ox-LDL group(P＜0.05).Compared with ox-LDL group,ox-LDL+shMALAT1 group the contents of total cholesterol and triglyceride,apoptosis,IL-6,TNFα and MCP-1,and the expression of NLRP3,RIPK1,caspase-8,p-p65 and SR-A all significantly decreased(P＜0.05).Conclusions MALAT1 may play an important role in ox-LDL-induced cell injury by regulating NLRP3 inflammatory corpuscles and related signal pathways.Knockdown of MALAT1 can significantly reduce the inflammatory reaction and cell damage in-duced by ox-LDL,which provides a potential target for the treatment of atherosclerosis.关键词
动脉粥样硬化/肺腺癌转移相关转录本1(MALAT1)/NOD样受体蛋白3(NLRP3)/炎性反应Key words
atherosclerosis/metastasis-associated lung adenocarcinoma transcript 1(MALAT1)/Nod-like receptor protein 3(NLRP3)/inflammation分类
医药卫生引用本文复制引用
宋宁,罗俊一,冀伟,李艳红,李晓梅,杨毅宁..敲降MALAT1减轻ox-LDL诱导的巨噬细胞炎性反应和细胞损伤[J].基础医学与临床,2026,46(3):352-358,7.基金项目
新疆维吾尔自治区自然科学基金青年科学基金(2022D01C765) （2022D01C765）
国家自然科学基金(8216020109,82460099) （8216020109,82460099）
