基础医学与临床2026，Vol.46Issue(3)：374-380,7.DOI:10.16352/j.issn.1001-6325.2026.03.0374
结直肠癌患者血浆外泌体中circGAPVD1高表达与病情相关
Elevated expression of circGAPVD1 in plasma-derived exosomes is correlated with disease progression in colorectal cancer patients
摘要
Abstract
Objective To explore the differential expression of circGAPVD1 derived from plasma exosomes in pa-tients with colorectal cancer(CRC),and to analyze its potential as a diagnostic marker for colorectal cancer.Methods A total of 111 CRC patients who underwent surgical treatment and were pathologically confirmed at the Affiliated Hospital of Xuzhou Medical University from January 2022 to August 2023 were enrolled,along with 85 healthy individuals from routine physical examinations as controls.Plasma exosomes were isolated and characterized using transmission electron microscopy(TEM),Western blot and nano-particle size analysis.The expression level of circGAPVD1 was detected by RT-qPCR.Receiver operating characteristic(ROC)curve analysis was performed to evaluate the diagnostic efficacy of circGAPVD1 for CRC.Results Compared with healthy controls,circGAPVD1 was significantly expressed higher in the plasma exosomes of patients with CRC(P＜0.01).The sensitivity of circ-GAPVD1 in the diagnosis was 77.5%with specificity as 76.5%.The area under ROC curve was 0.818 6,sugges-ting circGAPVD1 has robust diagnostic efficacy for CRC.In addition,related to the pathological data of patients with CRC,the lymph node metastasis and TNM staging showed a positive relation to high expression of circGAPVD1.Conclusions Plasma exosome-derived circGAPVD1 is a potential novel biomarker which support the clinical diag-nosis of CRC.关键词
结直肠癌/外泌体/环状RNA(circRNA)/诊断标志物Key words
colorectal cancer/exosome/circular RNA(circRNA)/diagnostic marker分类
医药卫生引用本文复制引用
詹森,刘宇杰,李天康,张冲,任泽强,张易..结直肠癌患者血浆外泌体中circGAPVD1高表达与病情相关[J].基础医学与临床,2026,46(3):374-380,7.基金项目
江苏省基础研究计划(自然科学基金)——优秀青年基金(BK20211606) （自然科学基金）
