江苏农业学报2026，Vol.42Issue(2)：392-398,7.DOI:10.3969/j.issn.1000-4440.2026.02.018
外源尿囊素对碱胁迫下菜豆种子萌发及抗性的影响
Effect of exogenous allantoin on common bean seed germination and re-sistance under alkali stress
摘要
Abstract
To explore the effects of allantoin on seed germination and physiological characteristics of common bean under alkali stress,the alkali-sensitive common bean variety Jinguan was used as the experimental material in this study.Based on the screening of sensitive alkali stress concentration and suitable allantoin soaking concentration,distilled water soaking+distilled water germination culture control(CK),distilled water soaking+40 mmol/L NaHCO3 germination cul-ture treatment(S),2 mmol/L allantoin soaking+40 mmol/L NaHCO3 germination culture treatment(S+All)were set.The contents of soluble protein,soluble sugar,proline and other osmotic adjustment substances in com-mon bean seeds,the activities of antioxidant enzymes such as superoxide dismutase(SOD),peroxidase(POD)and catalase(CAT),the production rate of superoxide anion free radical(O2·-)and the content of malondialdehyde(MDA)in cotyledons of common bean seeds were meas-ured at 12 h,24 h,36 h,48 h,60 h and 72 h after cul-ture.The results showed that the germination potential and germination rate of seeds treated with 2 mmol/L allantoin were 17.71%and 17.76%higher than those of distilled water treatment,respectively,which alleviated alkaline stress during the germination of common bean seeds.The soluble protein content,soluble sugar content,proline content in common bean seeds,and O2·-production rate and MDA content of the cotyledons of bean seeds treated with S at 12-72 h after culture were generally higher than those of CK,while the antioxidant enzyme activity was generally lower than that of CK in cotyle-dons of common bean.The soluble protein content,soluble sugar content,proline content in common bean seeds and an-tioxidant enzyme activity in cotyledons of common bean of S+All treatment were higher than those of S treatment,while the O2·-production rate and MDA content in cotyledons of common bean were lower than those of S treatment.The results of this study provide a basis for the application of allantoin in bean planting under alkaline stress.关键词
菜豆/种子萌发/碱胁迫/尿囊素Key words
common bean/seed germination/alkali stress/allantoin分类
农业科技引用本文复制引用
张晨,刘畅,冯国军,刘大军,闫志山,张泰峰,杨晓旭..外源尿囊素对碱胁迫下菜豆种子萌发及抗性的影响[J].江苏农业学报,2026,42(2):392-398,7.基金项目
黑龙江省自然科学基金优秀青年基金项目(YQ2024C046) （YQ2024C046）
黑龙江省"优秀青年教师基础研究支撑计划"项目(YQJH2024198) （YQJH2024198）
黑龙江省高等学校基本科研业务费项目(2024-KYYWF-0110) （2024-KYYWF-0110）
