扬州大学学报（农业与生命科学版）2026，Vol.47Issue(1)：55-64,10.DOI:10.16872/j.cnki.1671-4652.2026.01.007
表达PEDV RBD结构域的重组仙台病毒构建及其免疫原性评价
Construction of a recombinant Sendai virus expressing the PEDV RBD domain and its immunogenicity evaluation
摘要
Abstract
To construct a recombinant Sendai virus(SeV)expressing the receptor-binding domain(RBD)of porcine epidemic diarrhea virus(PEDV)and evaluate its immunogenicity,we designed a sequence-optimized PEDV spike(S)gene RBD domain(amino acids 507-757)engineered to contain four pairs of intramolecular disulfide bonds,referred to as RBD(8C).The optimized gene fragment was inserted into a SeV vector to generate the recombinant plasmid pBe-loBAC-SeV-PE-RBD(8C)-3Flag.Following virus rescue and characterization of its biological properties,the recombinant virus was used to immunize mice.PEDV-specific IgG and IgA antibody levels in serum and bronchoalveolar lavage fluid(BALF),serum neutralizing antibody titers,as well as T lymphocyte proliferation and T cell subset activation in lymph-oid tissues,were evaluated.The results demonstrated that the recombinant SeV specifically expressed the RBD(8C)pro-tein and exhibited a growth curve similar to that of wild-type SeV.Immunization with the recombinant virus effectively in-duced PEDV-specific IgG,IgA,and neutralizing antibodies in mice,significantly promoted PEDV-specific T cell prolifer-ation,and increased the proportions of CD4+and CD8+T cells in spleen and lymph nodes.In conclusion,the recombinant virus induced robust humoral,cellular,and mucosal immune responses in mice,providing experimental evidence to sup-port the development and optimization of PEDV vaccines.关键词
猪流行性腹泻病毒/RBD蛋白/仙台病毒载体/免疫原性评价Key words
PEDV/RBD protein/Sendai virus vector/immunogenicity evaluation分类
农业科技引用本文复制引用
魏丽萍,李嘉睿,潘朔楠,施开创,陈振海,牟春晓..表达PEDV RBD结构域的重组仙台病毒构建及其免疫原性评价[J].扬州大学学报（农业与生命科学版）,2026,47(1):55-64,10.基金项目
扬州市科技计划项目(YZ2024274)、扬州大学"青蓝工程"资助项目[扬大人资(2024)-22]、广西自然科学基金面上项目(2024GXNSFAA010108)、江苏高校优势学科建设工程资助项目(PAPD) （YZ2024274）
