江苏农业学报2026，Vol.42Issue(2)：217-224,8.DOI:10.3969/j.issn.1000-4440.2026.02.001
甘薯IbEFR基因克隆、生物信息学分析及表达模式
Cloning,bioinformatic analysis and expression patterns of IbEFR gene in sweet potato
摘要
Abstract
In this study,the IbEFR gene was cloned from Ipomoea batatas(L.)Lam.using RT-PCR technology.This gene has a full-length coding sequence(CDS)of 3 108 bp,encoding a protein of 1 035 amino acid residues.Domain analysis results showed that the IbEFR protein contained abundant leucine-rich repeat(LRR)sequences and a conserved serine/threonine kinase(S_TKc)domain.Sequence alignment results indicated that the amino acid sequence identity between the IbEFR protein and the Arabidopsis thaliana AtEFR protein was 44.76%.Phylogenetic tree analysis results revealed that the IbEFR gene had the closest genetic relationship with the EFR gene of tobacco.The prediction results of physicochemical properties indicated that the theoretical relative molecular mass of the IbEFR protein was approximately 112 605,and its theoretical isoelectric point was 5.8.In terms of amino acid composition,leucine content was 14.8%,serine con-tent was 10.0%,and glycine content was 8.1%,ranking as the top three,respectively.The instability index was 36.60,so it was determined to be a stable protein.Phosphorylation site prediction results showed that the IbEFR protein had the most serine sites(64),followed by threonine sites(19).Hydrophilicity and hydrophobicity prediction results indicated that IbEFR was a hydrophilic protein.Transmembrane domain prediction results showed that the IbEFR protein contained two obvious transmembrane domains,and the subcellular localiza-tion was most likely in the cytoplasm.Secondary structure prediction results demonstrated that the IbEFR protein was mainly composed of random coils,followed by α-helices and β-sheets.Expression pattern analysis results showed that the expression of the IbEFR gene could be induced and upregulated by abscisic acid(ABA),jasmonic acid(JA)and salicylic acid(SA),indicating that it may be involved in the hormone signal regulation pathway of sweet potato.The results of this study lay a foundation for the subsequent in-depth analysis of the function of the IbEFR gene.关键词
甘薯/抗病性/类受体激酶基因(EFR)/表达模式Key words
sweet potato/disease resistance/receptor-like kinase gene(EFR)/expression pattern分类
农业科技引用本文复制引用
杨冬静,高方园,马居奎,唐伟,陈晶伟,梁昭,佟聪,张成玲,孙厚俊..甘薯IbEFR基因克隆、生物信息学分析及表达模式[J].江苏农业学报,2026,42(2):217-224,8.基金项目
国家自然科学基金项目(32001599) （32001599）
国家现代农业产业技术体系项目(CARS-10) （CARS-10）
江苏省政府留学奖学金项目(JS-2014-120) （JS-2014-120）
