江苏农业学报2026，Vol.42Issue(2)：240-249,10.DOI:10.3969/j.issn.1000-4440.2026.02.003
芝麻NRAMP家族的全基因组鉴定及其在重金属胁迫下的表达分析
Genome-wide identification of the NRAMP family in Sesamum indicum L.and its expression analysis under heavy metal stress
摘要
Abstract
Natural resistance-associated macrophage proteins(NRAMP)are a class of metal ion transporters widely present in plants,mainly involved in the transport of divalent metal ions such as Cd2+and Mn2+.To explore the structure and function of the NRAMP family in Sesamum indicum L.,this study identified SiNRAMP family members using bioinfor-matics methods based on sesame whole-genome data,and systematically analyzed their protein and gene structural charac-teristics,as well as expression patterns under heavy metal stress.The results showed that the SiNRAMP family contained a total of six members,distributed on five chromosomes,with amino acid lengths ranging from 447 to 553.All SiNRAMP family members were localized to the cell membrane.The promoter regions of all SiNRAMP genes contained stress-responsive elements and various hormone-responsive ele-ments.The responses of SiNRAMP family members to dif-ferent heavy metal ion stresses were diverse.After 72 h of Cd2+treatment,the expression levels of SiNRAMP2,SiN-RAMP3,SiNRAMP5,and SiNRAMP6 were significantly higher than those in the control group(P<0.05),indicating that these NRAMP genes were collectively involved in the transmembrane transport of heavy metals.In addition,SiNRAMP3 could respond to both Cd2+and Cu2+stresses,suggesting its potential ability to transport multiple heavy metal ions.This study provides a theoretical basis for further elucidating the biological functions of sesame NRAMP genes and their molecular mechanisms in response to heavy metal stress.关键词
自然抗性相关巨噬蛋白/基因家族/芝麻/生物信息学分析Key words
natural resistance-associated macrophage protein/gene family/Sesamum indicum L./bioinformatic analysis分类
农业科技引用本文复制引用
李超琼,胡利宗,吴焕焕..芝麻NRAMP家族的全基因组鉴定及其在重金属胁迫下的表达分析[J].江苏农业学报,2026,42(2):240-249,10.基金项目
河南省科技研发计划联合基金项目(245101610086) （245101610086）
河南省科技攻关项目(202102110228) （202102110228）
周口师范学院2021年度校级中青年骨干教师项目(ZKNU202107) （ZKNU202107）
