菌物学报2026，Vol.45Issue(3)：43-59,17.DOI:10.13346/j.mycosystema.250188
三十五份吉林省猴头菇菌株的全基因组序列分析
Whole-genome analysis of 35 Hericium erinaceus strains from Jilin Province
摘要
Abstract
Currently,research on regional genetic diversity and population genetic structure of Hericium erinaceus remains relatively scarce.Additionally,the analysis of the genetic background of germplasm resources has largely relied on fragmented molecular markers,lacking a comprehensive landscape of genome-wide variations,and thereby hindering the progress of genetic improvement.In this study,whole-genome resequencing of 35 H.erinaceus germplasm resources from Jilin Province was performed.Based on variation data such as single nucleotide polymorphisms(SNPs)and small insertions/deletions(InDels),their genetic structure,evolutionary relationships,and diversity characteristics were systematically analyzed.Population genetic structure analysis revealed that the optimal number of clusters was K=2,with significant genetic isolation between the cultivated strains and wild populations(Fst=0.079 8-0.090 4),indicating that artificial selection has led to a significant deviation in the genomic genetic background of the cultivated populations from wild populations.Genetic distance and linkage disequilibrium(LD)analyses showed low genetic differentiation among wild populations(Fst=0.022 5-0.030 5)with an LD decay distance of 6-15 kb(r2<0.3),reflecting a coexistence pattern of geographical isolation and gene flow.Gene flow simulation confirmed weak gene flow to the cultivated strains only from the wild populations of Songshan Town and Changbai Mountain,with no genetic contribution from the wild populations of Huangsongdian Town and Manjiang Town.Principal component analysis(PCA)validated the geographical clustering of strains(the first three principal components explained 6.81%,3.35%,and 3.31%of the genetic variation,respectively)and simultaneously revealed that artificial selection had led to abnormal genomic genetic characteristics in the cultivated strains.This study provides crucial genomic data support for the precise conservation and targeted genetic improvement of H.erinaceus germplasm resources.By deciphering genetic patterns,it aids in broadening the genetic basis of cultivated populations and promotes sustainable industrial development.关键词
猴头菇/全基因组重测序/单核苷酸多态性/插入缺失变异/群体遗传结构Key words
Hericium erinaceus/whole-genome resequencing/single nucleotide polymorphism/insertion deletion variation/population genetic structure引用本文复制引用
马星月,王天娇,林晓龙,姚勋,郑佳澜,陈利丁,孙淑静..三十五份吉林省猴头菇菌株的全基因组序列分析[J].菌物学报,2026,45(3):43-59,17.基金项目
福建省农业引导性项目科技特派团服务食用菌重点产业链专项(2024N0055) This work was supported by the Agricultural Guiding Project,Science and Technology Commissioner Mission Special Project of Key Edible Mushroom Industry Chain of Fujian Province(2024N0055). （2024N0055）
