菌物学报2026，Vol.45Issue(3)：60-77,18.DOI:10.13346/j.mycosystema.250231
基于MNP标记的暗褐脉柄牛肝菌菌株鉴定与群体遗传研究
Strain identification and population genetic analysis of Phlebopus portentosus based on MNP markers
摘要
Abstract
Phlebopus portentosus,the first successfully domesticated and cultivated bolete species,has undergone rapid industrial expansion in recent years,highlighting the growing need for accurate strain identification and germplasm protection.In this study,whole-genome resequencing was performed on 66 strains of P.portentosus collected from various regions,resulting in the construction of a database of multiple nucleotide polymorphism(MNP)markers containing 306 loci.Population structure and kinship analyses based on both single nucleotide polymorphisms(SNPs)and MNPs consistently grouped the strains into two distinct genetic clusters.The MNP markers exhibited high resolution and stability across successive subculturing generations,enabling precise strain discrimination.Clonal identification criteria were established using replicate sequencing data of the same strains,with thresholds set at PI_HAT>0.95 and genetic distance(D)<0.01,while a genetic similarity(GS)value of 100%within the MNP dataset was validated as a robust indicator of strain identity.Additionally,machine learning approaches were employed to identify key MNP loci indicative of population divergence,offering valuable markers for varietal authentication and functional genomics.This work established MNP molecular marker database for P.portentosus and validated the potential applications in genetic resource conservation and molecular breeding,providing scientific support for variety protection and germplasm innovation.关键词
暗褐脉柄牛肝菌/群体结构/多核苷酸多态性/遗传相似度/菌株鉴定Key words
Phlebopus portentosus/population structure/multiple nucleotide polymorphism/genetic similarity/strain identification引用本文复制引用
孟国良,李嘉嘉,曹瑶,李凡,曾雨康,李荣春,董彩虹..基于MNP标记的暗褐脉柄牛肝菌菌株鉴定与群体遗传研究[J].菌物学报,2026,45(3):60-77,18.基金项目
云南省(珍稀食用菌)企业技术中心专项资金(JSJ2023016KJ) This work was supported by the Special Fund of Yunnan Province(Rare Edible Fungi)Enterprise Technology Center(JSJ2023016KJ). （珍稀食用菌）
