菌物学报2026，Vol.45Issue(3)：106-123,18.DOI:10.13346/j.mycosystema.250196
基于转录组学分析广叶绣球菌多糖改善小鼠骨骼肌糖代谢紊乱机制
Regulation mechanism of Sparassis latifolia polysaccharide improving glucose metabolism disorder in skeletal muscle of mice based on transcriptomics analysis
摘要
Abstract
The study was designed to investigate the improvement effect and molecular mechanism of Sparassis latifolia polysaccharide(SLPs)on glucose metabolism in skeletal muscle of mice with glucose metabolism disorder induced by high-fat diet combined with streptozotocin(STZ).The results showed that SLPs intervention could significantly reduce the impaired glucose tolerance and insulin resistance and increase skeletal muscle glycogen content and improve the morphological damage of skeletal muscle caused by glucose metabolism disorder.Transcriptome analysis identified a total of 965 differentially expressed genes(DEGs).GO annotation and enrichment analysis showed that these genes were mainly involved in tissue development,animal organ morphogenesis,small molecule metabolism,myofibril,glycogen binding,and organic acid binding.The enrichment analysis of KEGG further revealed that DEGs were mainly enriched in PI3k/Akt signal pathway,AGE-RAGE signal pathway related to diabetic complications,insulin resistance,AMPK signal pathway and other signal pathways.In addition,SLPs could regulate the expression of genes and proteins related to AMPK/SIRT1/PGC-1α signal pathway and the activities of key enzymes in skeletal muscle of mice to improve glucose metabolism disorder in skeletal muscle,protect skeletal muscle damage,and maintain the metabolic homeostasis of the body.This study provides a theoretical basis for the development of SLPs as functional food ingredients.关键词
广叶绣球菌多糖/糖代谢/骨骼肌/转录组学/AMPK/SIRT1/PGC-1α信号通路Key words
Sparassis latifolia polysaccharides/glucose metabolism/skeletal muscle/transcriptomics/AMPK/SIRT1/PGC-1α signaling pathway引用本文复制引用
王振,靳雯,魏欣,曹谨玲,云少君,程菲儿,冯翠萍..基于转录组学分析广叶绣球菌多糖改善小鼠骨骼肌糖代谢紊乱机制[J].菌物学报,2026,45(3):106-123,18.基金项目
山西省科技成果转化引导专项项目(202304021301055) （202304021301055）
山西省现代农业产业技术体系建设专项资金(2025CYJSTX09-02) （2025CYJSTX09-02）
山西省科技重大专项计划(202301140601015) （202301140601015）
山西省专利转化专项计划项目(202301009) This work was supported by the Science and Technology Achievement Transformation Guidance Special Project of Shanxi Province(202304021301055),the Earmarked Fund for Modern Agro-industry Technology Research System of Shanxi Province(2025CYJSTX09-02),the Major Special Science and Technology Projects of Shanxi Province(202301140601015),and the Special Project for Patent Commercialization of Shanxi Province(202301009). （202301009）
