临床与实验病理学杂志2026，Vol.42Issue(2)：177-182,189,7.DOI:10.13315/j.cnki.cjcep.2026.02.006
RAB2A通过AKT/CDC42信号通路调控肿瘤微环境对子宫颈癌细胞侵袭转移的影响
RAB2A regulates the effect of tumor microenvironment on invasion and metasta-sis of cervical cancer cells through AKT/CDC42 signaling pathway
摘要
Abstract
Objective To investigate the effect and its possible mechanism of RAB2A on the invasion and metas-tasis of cervical cancer cells by regulating tumor microenvironment via AKT/CDC42 signaling pathway.Methods Human normal cervical epithelial cells(H8),cervical cancer cells(Caski,HeLa,ME-180 and C33A)were cul-tured.RT-qPCR was used to assess RAB2A,AKT,and CDC42 mRNA levels,leading to the selection of ME-180 cells for subsequent experiments.These cells were divided into three groups:cervical cancer,RAB2A-knockdown(Low RAB2A),and RAB2A-knockdown with AKT activation(Low RAB2A+AKT activation).Protein expression levels of RAB2A,AKT,CDC42,HIF-1α,Notch-1,and Hes-1 were analyzed by Western blot.Immunofluorescence was used to detect HIF-1α,Notch-1,and Hes-1.Cell invasion and migration abilities were evaluated by Transwell assay and wound scratch test,respectively,scratch test to detect the migration ability of ME-180 cells.Results RAB2A,AKT,and CDC42 mRNA levels were significantly higher in cervical cancer cells(Caski,HeLa,ME-180 and C33A)compared to H8 cells(P<0.05),with the most pronounced increase observed in ME-180 cells.Protein levels of HIF-1α,Notch-1,and Hes-1 were also increased in ME-180 cells compared to H8 cells.Compared with the cervical cancer group,Low RAB2A led to decreased expression of AKT,CDC42,HIF-1α,Notch-1,and Hes-1 pro-teins(P<0.05),and significantly inhibited cell invasion and migration(P<0.05).The expressions of HIF-1α,Notch-1 and Hes-1 proteins in the Low RAB2A+AKT activation group were increased compared to the Low RAB2A group(P<0.05).Conclusion RAB2A can activate AKT/CDC42 signaling pathway to regulate tumor microenviron-ment and promote invasion and metastasis of cervical cancer cells.关键词
子宫颈肿瘤/RAB2A/AKT/CDC42通路/肿瘤微环境/侵袭/迁移Key words
cervical neoplasms/RAB2A/AKT/CDC42 pathway/tumor microenvironment/invade/migration分类
医药卫生引用本文复制引用
陈奕至,夏露花..RAB2A通过AKT/CDC42信号通路调控肿瘤微环境对子宫颈癌细胞侵袭转移的影响[J].临床与实验病理学杂志,2026,42(2):177-182,189,7.基金项目
首都卫生发展科研专项(2024-2-8011) Capital Health Development Research Special Project(2024-2-8011) （2024-2-8011）
