临床与实验病理学杂志2026，Vol.42Issue(2)：190-198,9.DOI:10.13315/j.cnki.cjcep.2026.02.008
子宫颈鳞状细胞癌中tRF-Glu38的表达及细胞增殖机制的研究
Expression of tRF-Glu38 in cervical squamous cell carcinoma and its mechanism on cell proliferation
摘要
Abstract
Objective This study aimed to investigate the expression of tRF-Glu38 in cervical squamous cell car-cinoma(CSCC)and its effect on CSCC cell proliferation.Methods The expression of tRF-Glu38 in CSCC tissues was detected by qRT-PCR.Cell proliferation was assessed using CCK-8 assay and colony formation assay.Down-stream target genes of tRF-Glu38 were predicted by bioinformatics and validated by dual-luciferase assay.Rescue ex-periments were conducted to verify the regulation of downstream target genes by tRF-Glu38 and their effects on cell pro-liferation.The impact of tRF-Glu38 on the growth and proliferation of transplanted tumors in nude mice was evalu-ated.Results The expression of tRF-Glu38 in 82 CSCC tissues(8.12±2.16)was significantly higher than that in adjacent normal tissues(3.32±1.06).tRF-Glu38 expression was closely associated with tumor size and differentia-tion.CCK-8 assays showed that overexpression of tRF-Glu38 promoted the proliferation of C33A and SiHa cells(both P<0.05),whereas knockdown of tRF-Glu38 inhibited their proliferation(both P<0.05).Colony formation assays re-vealed that the numbers of colonies in SiHa and C33A cells overexpressing tRF-Glu38 were 33.37±2.72 and 32.60±1.80,respectively,significantly higher than those in the control group(17.13±1.82 and 17.20±1.20)(both P<0.05).In the tRF-Glu38 knockdown group,colony numbers decreased to 11.53±1.04 and 10.20±1.30,respec-tively,significantly lower than the control(18.57±1.33 and 17.17±2.12)(both P<0.05).In vivo,tRF-Glu38 pro-moted the growth of CSCC xenografts in nude mice.Dual-luciferase assays confirmed that tRF-Glu38 bound to the 3′untranslated region of TP53I11,suppressing its expression and thereby promoting CSCC cell proliferation.Rescue ex-periments showed that overexpression of tRF-Glu38 decreased TP53I11 protein levels and increased NF-κB levels,whereas knockdown had the opposite effects.Further colony formation assays indicated that the tRF-Glu38/TP53I11/NF-κB signaling pathway promoted cervical cancer cell colony formation.Conclusion tRF-Glu38 is a novel oncogene in CSCC that promotes NF-κB expression by inhibiting TP53I11.The tRF-Glu38/TP53I11/NF-κB signaling pathway contributes to CSCC cell proliferation and growth.关键词
子宫颈鳞状细胞癌/tRF-Glu38/TP53I11/细胞增殖/NF-κBKey words
cervical squamous cell carcinoma/tRF-Glu38/TP53I11/cell proliferation/NF-κB分类
医药卫生引用本文复制引用
冯梓嫣,王成海..子宫颈鳞状细胞癌中tRF-Glu38的表达及细胞增殖机制的研究[J].临床与实验病理学杂志,2026,42(2):190-198,9.基金项目
江苏省卫健委科研项目(Z2022046)、江苏省大学生创新训练计划(S202511117021、S202511117022) Research Project of Health Commission of Jiangsu Province(Z2022046) （Z2022046）
Jiangsu Provincial Col-lege Student Innovation Training Program(S202511117021,S202511117022) （S202511117021,S202511117022）
