南京中医药大学学报2026，Vol.42Issue(2)：182-194,13.DOI:10.14148/j.issn.1672-0482.2026.0182
栝楼块根抗性淀粉-羧甲基壳聚糖复合物对肥胖小鼠血脂代谢及肠道菌群的调节作用
Trichosanthes Kirilowii Root Resistant Starch-Carboxymethyl Chitosan Complex Regulates Blood Lipid Metabolism and Intestinal Flora in Obese Mice
摘要
Abstract
OBJECTIVE To investigate the regulatory effects of Trichosanthes kirilowii root resistant starch-carboxymethyl chi-tosan complex(TKR-RS-CMC)on lipid metabolism and intestinal flora in obese mice by adding it to their diet.METHODS The TKR-RS-CMC was premix-mixed with basic diet and fed to Lepob/ob mice.The changes of body weight and blood glucose were ob-served,liver weight was determined,and pathological status of liver in each group was analyzed.Serum triglyceride(TG),total choles-terol(TC),insulin(INS),low density lipoprotein cholesterol(LDL-C),high density lipoprotein cholesterol(HDL-C),alanine aminotrans-ferase(ALT/GPT),aspartate aminotransferase(AST/GOT),malondialdehyde(MDA)and superoxide dismutase(SOD)in liver tissue were detected by biochemical and ELISA.Mice colon contents were sequenced with 16S rDNA in high throughput,and mice serum was ana-lyzed by UPLC-Q-TOF/MS.RESULTS Lepob/ob model mice showed dyslipidemia,which was represented by increased contents of TC,TG and LDL-C(P<0.001)and decreased content of HDL-C(P<0.001).TKR-RS-CMC could significantly improve the dyslipid-emia by reducing the contents of TC and LDL-C(P<0.001)and increasing the content of HDL-C(P<0.001)in obese mice.Lepob/ob model mice showed disorder of oxygen free radical metabolism,which was represented by decreased SOD activity(P<0.001)and in-creased MDA content(P<0.001),and MDA content decreased significantly(P<0.001)after TKR-RS-CMC was given in the diet.The results of oil red O staining and HE staining showed that the liver of Lepob/ob model mice had pathological changes,and the liver patho-logical sections of the administration group showed that the degree of damage was reduced.Obesity led to intestinal flora disorder,in which the relative abundance of Prevotellaceae_UCG-001 was significantly reduced(P<0.01),and the relative abundance of the above flora was reversed to that of the control group after TKR-RS-CMC was given in the diet.Compared with the model group,the relative abundance of Erysipelatoclostridium in the TKR-RS-CMC high-dose group was significantly decreased(P<0.05),while the relative abundance of Akkermansia,Muribaculum,Bifidobacterium and Ileibacterium was significantly increased(P<0.05).TKR-RS-CMC could significantly regulate the disregulated fatty acids and phospholipids in the serum of obese mice,involving the glycero phospho-lipid metabolic pathway,the mutual conversion pathway between pentose and glucuronic acid,and the metabolic pathway of cysteine and methionine.Spearman correlation analysis showed that metabolites involved in glycero phospholipid metabolic pathway were sig-nificantly associated with different intestinal flora.The probiotic Akkermansia was significantly negatively correlated with LysoPC(P-18∶0/0∶0),LysoPC(P-16∶0/0∶0)and other phospholipid metabolites.CONCLUSION TKR-RS-CMC can regulate the intestinal flora of obese mice and balance the disorder of blood lipid metabolism,suggesting that the compound could be used as a potential di-etary supplement to ameliorate obesity.关键词
栝楼块根/抗性淀粉/淀粉-壳聚糖复合物/肥胖/血脂代谢/肠道菌群Key words
Trichosanthes kirilowii root/resistant starch/starch-carboxymethyl chitosan complex/obesity/blood lipid metabolism/intestinal flora分类
医药卫生引用本文复制引用
王甜,代易,丁桂智,张晔钧,肖平,郭盛,潘欣,段金廒,刘培..栝楼块根抗性淀粉-羧甲基壳聚糖复合物对肥胖小鼠血脂代谢及肠道菌群的调节作用[J].南京中医药大学学报,2026,42(2):182-194,13.基金项目
国家自然科学基金面上项目(82274404) （82274404）
"六大人才高峰"高层次人才C类(JNHB-066) （JNHB-066）
江苏省"333工程"第三层次培养对象 （）
江苏省高校自然科学基金重大项目(23KJA360005) （23KJA360005）
国家中医药管理局高水平中医药重点学科建设项目——中药资源化学(zyyzdxk-2023083) （zyyzdxk-2023083）
国家中医药管理局创新团队及人才培养计划(ZYYCXTD-D-202005) （ZYYCXTD-D-202005）
