药学研究2026，Vol.45Issue(2)：121-129,140,10.DOI:10.13506/j.cnki.jpr.2026.02.001
基于指纹图谱、网络药理学和多指标定量技术筛选荷丹片治疗高脂血症的质量标志物
Screening of quality markers for Hedan Tablets in the treatment of hyperlipidemia based on fingerprint,network pharmacology and multi-constituent quantification
摘要
Abstract
Objective Based on the concept of quality marker(Q-marker)in traditional Chinese medicine(TCM),the fingerprint,network pharmacology and multi-constituent quantification were used to screen the potential Q-Marker of Hedan Tablets(HDT)for the treatment of hyperlipidemia.Methods Firstly,the HPLC fingerprint of HDT was established and the differential markers were analyzed by chemical pattern recognition.Secondly,by using network pharmacology,the potential targets and pathways of HDT in treatment of hyperlipidemia were predicted,and the potential Q-Marker then screened out according to the degree value.Finally,a UHPLC-based multi-constituent quantification was established to determine the content of the predicted landmark components.Results The fingerprint was established and 21 common peaks were detected,from which 10 differential markers were selected by chemical pattern recognition and confirmed by reference substance chromatography.Utilizing network pharmacology analysis,the above 10 differential markers exert lipid-lowering effects by acting on Akt1,TNF,PPARG,MMP9,TP53,HMGCR and other targets to modulate the signaling pathways of endocrine resistance,insulin resistance,lipids and atherosclerosis.And then 6 core active ingredients,including sennoside A,rosmarinic acid,quercetin-3-O-glucuronide,hyperoside,psoralenoside and nuciferine were screened as the potential Q-Markers based on the degree value,with the content of 0.01～0.09,0.02～0.27,0.21～4.30,0.09～1.71,0.06～0.54,0.11～0.60 mg per tablet,respectively.Conclusion The above 6 core active ingredients were consistent with the screening principles of TCM Q-Markers,such as the compatibility environment,effectiveness,specificity and measurability,and could be used as potential Q-Markers for HDT in the treatment of hyperlipidemia.关键词
荷丹片/高脂血症/质量标志物/网络药理学/化学模式识别/指纹图谱/多指标定量Key words
Hedan Tablets/Hyperlipidemia/Quality markers/Network pharmacology/Chemical pattern recognition/Fingerprint/Multi-constituent quantification分类
医药卫生引用本文复制引用
曹艺凡,夏林波,谢燕,李汉月,邹烨,邓仕任..基于指纹图谱、网络药理学和多指标定量技术筛选荷丹片治疗高脂血症的质量标志物[J].药学研究,2026,45(2):121-129,140,10.基金项目
辽宁省自然科学基金项目(No.20170540617) （No.20170540617）
辽宁中医药大学大学生创新创业训练计划项目(No.202310162003) （No.202310162003）
