药学研究2026，Vol.45Issue(2)：130-133,149,5.DOI:10.13506/j.cnki.jpr.2026.02.002
棕矢车菊素对新型冠状病毒RNA聚合酶的抑制活性
Inhibitory activity of jaceosidin on the SARS-CoV-2 RNA polymerase
摘要
Abstract
Objective To explore the inhibitory activity of the flavonoid jaceosidin against the RNA polymerase of the novel coronavirus.Methods Molecular docking was first employed to investigate the binding mode of jaceosidin at the active site of RNA polymerase,to determine whether jaceosidin could stably bind to the NTPs channel and obstruct the entry of substrates.Subsequently,the inhibitory pharmacological activity of jaceosidin against RNA polymerase was evaluated using a cell-based assay system for the catalytic reaction of the novel coronavirus RNA polymerase.Results Jaceosidin exhibited favorable binding to the active site of RNA polymerase,occupying the NTPs channel and hindering the entry of nucleotides,thereby achieving pharmacological action to inhibit RNA polymerase-mediated nucleic acid replication.The results of pharmacological experiments at the cellular level demonstrated that jaceosidin possesses inhibitory activity against RNA polymerase,albeit with weaker activity compared to remdesivir.However,jaceosidin exhibited lower cytotoxicity compared to remdesivir.Conclusion The findings of this study indicate that jaceosidin exhibits inhibitory activity against RNA polymerase,providing a potential reference for the development of anti-novel coronavirus drugs.关键词
新型冠状病毒/RNA聚合酶/活性位点/棕矢车菊素/分子对接/活性测试Key words
SARS-CoV-2/RNA polymerase/Active site/Jaceosidin/Molecular dock/Activity test分类
医药卫生引用本文复制引用
邸琨,刘琪,吴巧娜,刘晶磊,王宇佳,苏彦雷,孙殿兴,赵建元..棕矢车菊素对新型冠状病毒RNA聚合酶的抑制活性[J].药学研究,2026,45(2):130-133,149,5.基金项目
河北省医学科研课题项目(No.20241370) （No.20241370）
