药学研究2026，Vol.45Issue(2)：134-140,7.DOI:10.13506/j.cnki.jpr.2026.02.003
刺五加提取物对α-葡萄糖苷酶的抑制作用及酶动力学分析
Inhibitory effect of α-glucosidase by Eleutherococcus senticosus extract and enzyme kinetics analysis
摘要
Abstract
Objective To investigate the inhibitory effect and underlying mechanism of Eleutherococcus senticosus ex-tract on α-glucosidase.Methods The half-maximal inhibitory concentration(IC50)was determined using in vitro enzyme inhibition assays.Enzyme kinetics and fluorescence spectroscopy were employed to analyze the inhibition type,interaction mechanism,and thermodynamic parameters.Results E.senticosus extract exhibited a dose-dependent inhibitory effect on α-glucosidase,with an IC50 value of 0.86 mg·mL-1.The inhibition was characterized as predominantly mixed-type with a non-competitive component.Fluorescence spectroscopy revealed that the extract induced conformational changes in the enzyme through dynamic quenching.Thermodynamic analysis indicated that hydrophobic interactions were the main driving force.Conclusion E.senticosus extract inhibits α-glucosidase through conformational modulation and hydrophobic interactions,suggesting its potential application in blood glucose regulation.关键词
刺五加/α-葡萄糖苷酶/动力学分析/荧光光谱/降糖活性Key words
Eleutherococcus senticosus/α-glucosidase/Kinetic analysis/Fluorescence spectroscopy/Hypoglycemic activ-ity分类
医药卫生引用本文复制引用
闫琳,倪天凤,陈小强,张莹..刺五加提取物对α-葡萄糖苷酶的抑制作用及酶动力学分析[J].药学研究,2026,45(2):134-140,7.基金项目
东北林业大学大学生创新训练计划项目(No.202310225478) （No.202310225478）
