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咖啡S-腺苷甲硫氨酸合成酶基因CaSAMS3的克隆及表达模式分析

王曦奥王晓阳 SANCHEZWilliam Solano 闫林廖振阳安娜胡丽松黄丽芳赵新传熊文艳庞永青王韫镭

热带作物学报2026，Vol.47Issue(2)：302-312,11.

热带作物学报2026，Vol.47Issue(2)：302-312,11.DOI:10.3969/j.issn.1000-2561.2026.02.003

咖啡S-腺苷甲硫氨酸合成酶基因CaSAMS3的克隆及表达模式分析

Molecular Cloning and Expression Pattern Analysis of S-adenosylme-thionine Synthase Gene CaSAMS3 in Coffee

王曦奥 ¹王晓阳 ¹SANCHEZWilliam Solano ²闫林 ¹廖振阳 ³安娜 ¹胡丽松 ³黄丽芳 ¹赵新传 ⁴熊文艳 ¹庞永青 ¹王韫镭⁵

作者信息

1. 中国热带农业科学院香料饮料研究所,海南万宁 571533||咖啡海南省工程研究中心,海南万宁 571533||热带香料饮料国际联合研究中心,海南万宁 571533||农业部香辛饮料作物遗传资源利用重点实验室,海南万宁 571533||海南省热带香辛饮料作物遗传改良与品质调控重点实验室,海南万宁 571533
2. 热带香料饮料国际联合研究中心,海南万宁 571533||热带农业研究与高等教育中心,哥斯达黎加图里亚尔巴 30501
3. 中国热带农业科学院香料饮料研究所,海南万宁 571533||咖啡海南省工程研究中心,海南万宁 571533||热带香料饮料国际联合研究中心,海南万宁 571533||农业部香辛饮料作物遗传资源利用重点实验室,海南万宁 571533||海南省热带香辛饮料作物遗传改良与品质调控重点实验室,海南万宁 571533||热带作物生物育种全国重点实验室,海南三亚 572024
4. 云南农业大学热带作物学院,云南普洱 665000
5. 中国热带农业科学院香料饮料研究所,海南万宁 571533||农业部香辛饮料作物遗传资源利用重点实验室,海南万宁 571533||海南省热带香辛饮料作物遗传改良与品质调控重点实验室,海南万宁 571533
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摘要

Abstract

S-adenosylmethionine synthase(SAMS)is a key enzyme catalyzing the synthesis of S-adenosylmethionine(SAM,S-adenosylmethionine),widely involved in various biological processes in plants,such as epigenetic regulation,oxidative stress,and growth and development.In this study,the full-length cDNA sequence of CaSAMS3 was successfully cloned from coffee,and the physicochemical properties,protein structure,conserved domains,and evolutionary characteristics of the en-coded CaSAMS3 protein were analyzed.Through cis-acting element analysis and protein interaction network prediction,and the metabolic processes involving CaSAMS3 were explored,and its expression pattern was analyzed in combination with qPCR and subcellular localization studies.The results showed that the open reading frame length of CaSAMS3 was 1173 bp,encoding a hydrophilic protein(molecular weight 42.57 kDa,theoretical isoelectric point 6.07),with a secondary structure mainly composed of α-helices and irregular curls,and located on cell membrane and nucleus.The protein contained a typical SAMS conserved domain and had the highest homology with SAMS in kiwifruit.Its promoter contained response elements related to light,hormones,stress,and growth and development,including MYB and MYC binding sites.CaSAMS3 exhibited tissue-specific expression,and its expression trend during developmental stages was consistent with accumulation pattern of caffeine.This study would provide an important theoretical basis for elucidating the biological function of CaSAMS3 in the regulation of caffeine synthesis,and provides a potential target for improving coffee quality and resistance.

关键词

咖啡/咖啡因/S-腺苷甲硫氨酸合成酶/基因克隆/表达模式

Key words

coffee/caffeine/S-adenosylmethionine synthase/gene cloning/expression pattern

分类

农业科技

引用本文复制引用

王曦奥,王晓阳,SANCHEZWilliam Solano,闫林,廖振阳,安娜,胡丽松,黄丽芳,赵新传,熊文艳,庞永青,王韫镭..咖啡S-腺苷甲硫氨酸合成酶基因CaSAMS3的克隆及表达模式分析[J].热带作物学报,2026,47(2):302-312,11.

基金项目

国家现代农业产业技术体系项目(No.CARS-11) （No.CARS-11）

海南省自然科学基金青年基金项目(No.324QN309). （No.324QN309）

热带作物学报

ISSN：1000-2561

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