热带作物学报2026，Vol.47Issue(2)：336-349,14.DOI:10.3969/j.issn.1000-2561.2026.02.006
美花卷瓣兰与长花石豆兰的叶绿体基因组比较和系统发育分析
Comparative Analysis and Phylogenetic Study of the Chloroplast Genomes of Bulbophyllum rothschildianum and Bulbophyllum lon-gissimum
摘要
Abstract
The aim of the study was to characterize the chloroplast genome structure and phylogenetic relationships within the genus Bulbophyllum.We sequenced,assembled,and annotated the chloroplast genomes of B.rothschildianum and B.longissimum for the first time.Comparative analyses of codon usage bias,repeat sequences,and sequence varia-tion were then conducted with the available chloroplast genomes from four previously published Bulbophyllum species.The chloroplast genome of B.rothschildianum and B.longissimum was 148 447 bp and 151 095 bp,respectively,both showing a typical circular quadripartite structure containing 126 and 129 functional genes.Codons encoding leucine exhibited the strongest usage bias,with codons ending in A/U being particularly abundant.SSR analysis revealed mo-nonucleotide repeats(predominantly A/T type)were most abundant,and palindromic repeats dominated among long repeats.Highly variable regions were mainly located in non-coding regions and selected coding genes(e.g.,matK and ycf1),with evident expansion and contraction at IR/SC boundaries.Phylogenetic analysis showed that the 32 Bulbo-phyllum species formed a strongly supported clade and exhibited clear geographic differentiation into Asian and Central and South American lineages,with B.rothschildianum and B.longissimum clustering together within the Asian lineage and being closely related.The study would provide molecular insights into resource development,genetic conservation,and systematic research in the genus Bulbophyllum.关键词
美花卷瓣兰/长花石豆兰/叶绿体基因组/比较基因组学/系统发育分析Key words
Bulbophyllum rothschildianum/Bulbophyllum longissimum/chloroplast genome/comparative genomics/phylogenetic analysis分类
农业科技引用本文复制引用
邹婉霞,欧阳勤森,田海娟,郝静静,卓书斌..美花卷瓣兰与长花石豆兰的叶绿体基因组比较和系统发育分析[J].热带作物学报,2026,47(2):336-349,14.基金项目
东莞市社会发展科技项目(No.20231800940762). （No.20231800940762）
