四川大学学报（自然科学版）2026，Vol.63Issue(2)：297-307,11.DOI:10.19907/j.0490-6756.250338
Notch通路基因Djaph-1、Djcsl与Djnumb在涡虫再生中的功能研究
Functional roles of the Notch pathway genes Djaph-1,Djcsl and Djnumb in planarian regeneration
摘要
Abstract
The Notch signaling pathway is a conserved intercellular communication mechanism that plays a crucial role in tissue development and homeostasis.To investigate its functions in tissue regeneration and stem cell regulation,planarians were used as an experimental model.Homologous of the Notch signaling pathway genes aph-1,csl,and numb were identified in Dugesia japonica and their biological functions during planarian regeneration were characterized.The results showed that Djaph-1,Djcsl,and Djnumb mRNA were broadly expressed in whole planarians and were highly enriched in the regenerating blastema.Knock-down of Djaph-1,Djcsl,and Djnumb led to abnormal phenotypes such as black spots along the body axis and asymmetric eye spots,as well as defects in central nervous system regeneration.These phenotypes were ac-companied by altered cell proliferation dynamics at different regeneration stages and chenges in the expression of eye-and epidermis-related genes.Further studies demonstrated that Djaph-1 and Djcsl positively regulate Notch signaling,whereas Djnumb functions as a negative regulator.Collectively,these results suggest that Djaph-1,Djcsl,and Djnumb are key regulators of stem cell proliferation and differentiation and play crucial roles in planarian regeneration.关键词
涡虫/再生/干细胞/增殖/Notch通路基因Key words
planarian/regeneration/stem cells/proliferation/Notch signaling pathway genes分类
生物科学引用本文复制引用
张文文,金白洁,孙丹丹,郭伟云,霍金瑞,董自梅,陈广文,刘德增..Notch通路基因Djaph-1、Djcsl与Djnumb在涡虫再生中的功能研究[J].四川大学学报（自然科学版）,2026,63(2):297-307,11.基金项目
国家自然科学基金项目(32270501,32470463) （32270501,32470463）
河南省重大公益项目(201300311700) （201300311700）
河南省高等学校重点科研项目(26A180012) （26A180012）
